Mapping chromatin modifications at the single cell level

被引:41
作者
Ludwig, Connor H. [1 ]
Bintu, Lacramioara [1 ]
机构
[1] Stanford Univ, Dept Bioengn, Shriram Ctr, 443 Via Ortega,Rm 042, Stanford, CA 94305 USA
来源
DEVELOPMENT | 2019年 / 146卷 / 12期
基金
美国国家卫生研究院;
关键词
DNA methylation; Chromatin regulation; Gene regulation; Histone modifications; Single cell detection; DNA METHYLATION; HISTONE MODIFICATIONS; CHROMOSOME-WIDE; EARLY EMBRYOS; BISULFITE; METHYLOME; MOLECULE; SEQ; 5-METHYLCYTOSINE; RESOLUTION;
D O I
10.1242/dev.170217
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Understanding chromatin regulation holds enormous promise for controlling gene regulation, predicting cellular identity, and developing diagnostics and cellular therapies. However, the dynamic nature of chromatin, together with cell-to-cell heterogeneity in its structure, limits our ability to extract its governing principles. Single cell mapping of chromatin modifications, in conjunction with expression measurements, could help overcome these limitations. Here, we review recent advances in single cell-based measurements of chromatin modifications, including optimization to reduce DNA loss, improved DNA sequencing, barcoding, and antibody engineering. We also highlight several applications of these techniques that have provided insights into cell-type classification, mapping modification co-occurrence and heterogeneity, and monitoring chromatin dynamics.
引用
收藏
页数:16
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