Nitric oxide and liver microcirculation during autoregulation and haemorrhagic shock in rabbit model

被引:12
作者
Lhuillier, F.
Robert, M. -O.
Crova, P.
Goudable, J.
Arnal, F.
Cespuglio, R.
Annat, G.
Viale, J. -P.
机构
[1] Hop Croix Rousse, Dept Anesthesie Reanimat, F-69317 Lyon 04, France
[2] UPRES EA 1896, F-69008 Lyon, France
[3] INSERM U480, F-69008 Lyon, France
关键词
complications; haemorrhage; liver; blood flow; microcirculation; pharmacology; nitric oxide;
D O I
10.1093/bja/ael097
中图分类号
R614 [麻醉学];
学科分类号
100217 ;
摘要
Background. Direct evidence of nitric oxide (NO) involvement in the regulation of hepatic microcirculation is not yet available under physiological conditions nor in haemorrhagic shock. Methods. A laser Doppler flowmetry was used to measure liver perfusion index and a specific NO-sensitive electrode was inserted into liver parenchyma of anaesthetized rabbits. Hepatic autoregulation during moderate hypovolaemia {mean arterial pressure at 50 mm Hg without liver perfusion alteration; blood withdrawal 17.7 (4.2) ml [mean (SD)]} or haemorrhagic shock [mean arterial pressure at 20 mm Hg associated with liver perfusion impairment and lactic acidosis; blood withdrawal 56.0 (6.8) ml] were investigated over 60 min and were followed by a rapid infusion of the shed blood. Involvement of NO synthases was evaluated using a non-specific inhibitor, NAPNA (Nw-nitro-L-arginine P-nitro-anilide). Conclusions. NO plays a physiological role in the liver microcirculation during autoregulation. Its production is enzyme-dependent. Conversely, haemorrhagic shock induces a rapid increase in hepatic NO that is at least partially enzyme-independent.
引用
收藏
页码:137 / 146
页数:10
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