Artemisia scoparia Enhances Adipocyte Development and Endocrine Function In Vitro and Enhances Insulin Action In Vivo

被引:23
作者
Richard, Allison J. [1 ]
Fuller, Scott [1 ]
Fedorcenco, Veaceslav [1 ]
Beyl, Robbie [1 ]
Burris, Thomas P. [2 ]
Mynatt, Randall [1 ]
Ribnicky, David M. [3 ]
Stephens, Jacqueline M. [1 ]
机构
[1] Louisiana State Univ, Pennington Biomed Res Ctr, Baton Rouge, LA 70808 USA
[2] St Louis Univ, Sch Med, Dept Pharmacol & Physiol Sci, St Louis, MO 63104 USA
[3] Rutgers State Univ, Dept Plant Biol & Pathol, New Brunswick, NJ 08903 USA
基金
美国国家卫生研究院;
关键词
ACTIVATED PROTEIN-KINASE; NECROSIS-FACTOR-ALPHA; ADIPOSE-TISSUE; SKELETAL-MUSCLE; PPAR-GAMMA; METABOLIC SYNDROME; DIABETES-MELLITUS; 3T3-L1; ADIPOCYTES; HEPATIC INSULIN; GENE-EXPRESSION;
D O I
10.1371/journal.pone.0098897
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background: Failure of adipocytes to expand during periods of energy excess can result in undesirable metabolic consequences such as ectopic fat accumulation and insulin resistance. Blinded screening studies have indicated that Artemisia scoparia (SCO) extracts can enhance adipocyte differentiation and lipid accumulation in cultured adipocytes. The present study tested the hypothesis that SCO treatment modulates fat cell development and function in vitro and insulin sensitivity in adipose tissue in vivo. Methods: In vitro experiments utilized a Gal4-PPAR gamma ligand binding domain (LBD) fusion protein-luciferase reporter assay to examine PPAR gamma activation. To investigate the ability of SCO to modulate adipogenesis and mature fat cell function in 3T3-L1 cells, neutral lipid accumulation, gene expression, and protein secretion were measured by Oil Red O staining, qRT-PCR, and immunoblotting, respectively. For the in vivo experiments, diet-induced obese (DIO) C57BL/6J mice were fed a high-fat diet (HFD) or HFD containing 1% w/w SCO for four weeks. Body weight and composition, food intake, and fasting glucose and insulin levels were measured. Phospho-activation and expression of insulin-sensitizing proteins in epididymal adipose tissue (eWAT) were measured by immunoblotting. Results: Ethanolic extracts of A. scoparia significantly activated the PPAR gamma LBD and enhanced lipid accumulation in differentiating 3T3-L1 cells. SCO increased the transcription of several PPAR gamma target genes in differentiating 3T3-L1 cells and rescued the negative effects of tumor necrosis factor alpha on production and secretion of adiponectin and monocyte chemoattractant protein-1 in fully differentiated fat cells. DIO mice treated with SCO had elevated adiponectin levels and increased phosphorylation of AMPK alpha in eWAT when compared to control mice. In SCO-treated mice, these changes were also associated with decreased fasting insulin and glucose levels. Conclusion: SCO has metabolically beneficial effects on adipocytes in vitro and adipose tissue in vivo, highlighting its potential as a metabolically favorable botanical supplement.
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页数:8
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