Direct Analysis of Succinic Acid Fermentation Broth by 1H Diffusion-Ordered NMR Spectroscopy and Quantitative 1H NMR Technique

The diffusion-ordered NMR spectroscopy (DOSY) protocol was first introduced to analyze directly the mixture samples of succinic acid fermentation broth. The NMR signals of the model mixtures and the practical mixture were facilely separated, and each component was fully assigned in the diffusion dimension of DOSY spectra. The composition of succinic acid fermentation broth could be rapidly determined according to the DOSY experiments. In contrast, this qualitative analysis could not be implemented through ion chromatography technique. On the basis of DOSY experimental results, the quantitative H-1 NMR (qHNMR) technique was further used to determinate successfully the components of one mixture sample of practical fermentation broth, which agreed well with the high-performance liquid chromatographic experimental result. The quantitative analysis results demonstrate that the DOSY technique and the qHNMR technique can determination of the product quality of succinic acid fermentation broth.