The silkrose of Bombyx mori effectively prevents vibriosis in penaeid prawns via the activation of innate immunity

被引：22

作者：

Ali, Muhammad Fariz Zahir ^{[1
]}

Yasin, Indri Afriani ^{[1
]}

Ohta, Takashi ^{[2
]}

Hashizume, Atsushi ^{[1
]}

Ido, Atsushi ^{[2
]}

Takahashi, Takayuki ^{[1
]}

Miura, Chiemi ^{[1
,3
]}

Miura, Takeshi ^{[1
]}

机构：

[1] Ehime Univ, Grad Sch Agr, 3-5-7 Tarumi, Matsuyama, Ehime 7908566, Japan

[2] Ehime Univ, South Ehime Fisheries Res Ctr, 1289-1 Funakoshi, Ainan, Ehime 7984292, Japan

[3] Hiroshima Inst Technol, Fac Environm Studies, Dept Global Environm Studies, Saeki Ku, 2-1-1 Miyake, Hiroshima 7315193, Japan

来源：

SCIENTIFIC REPORTS | 2018年 / 8卷

基金：

日本科学技术振兴机构; 日本学术振兴会;

关键词：

TOLL RECEPTOR; LITOPENAEUS-VANNAMEI; KURUMA SHRIMP; GENE-TRANSFER; RECOGNITION; SILKWORM; PEPTIDE; FISH; IDENTIFICATION; ALGINOLYTICUS;

D O I：

10.1038/s41598-018-27241-3

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We previously identified novel bioactive polysaccharides from Bactrocera cucurbitae and Antheraea yamamai that activate innate immunity in RAW264 murine macrophages. However, in terms of potential applications in the cultivation of prawns, there were problems with the availability of these insects. However, we have now identified a polysaccharide from Bombyx mori that activates innate immunity in RAW264 cells and penaeid prawns. This purified polysaccharide, termed silkrose of B. mori (silkrose-BM), has a molecular weight of 1,150,000 and produces a single symmetrical peak on HPLC. Eight of nine constitutive monosaccharides of silkrose-BM are concomitant with dipterose of B. cucurbitae (dipterose-BC) and silkrose of A. yamamai (silkrose-AY). The major differences are found in the molar ratios of the monosaccharides. Silkrose-BM is approximately 500-fold less potent than silkrose-AY (EC50: 2.5 and 0.0043 mu g/mL, respectively) in a nitrite oxide (NO) production assay using RAW264 cells. However, the maximum NO production for silkrose-BM and AY were comparable and higher than that of the lipopolysaccharide of Escherichia coli. The survival of penaeid prawns (Litopenaeus vannamei and Marsupenaeus japonicus) after infection with Vibrio penaecida was significantly improved by both dietary silkrose-BM and B. mori pupae. This suggests that silkrose-BM effectively prevents vibriosis in penaeid prawns via the activation of innate immunity.

引用

页数：8

共 56 条

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