Isolation and Characterization of 14 Polymorphic Microsatellite Loci in the Big-Headed Turtle (Platysternon megacephalum)

被引：6

作者：

Hua, Liushuai ^{[1
]}

Wang, Fumin ^{[2
]}

Gong, Shiping ^{[1
]}

Ge, Yan ^{[1
]}

Chen, Guoling ^{[1
]}

Chen, Jinping ^{[1
]}

机构：

[1] South China Inst Endangered Anim, Guangdong Prov Publ Lab Wild Anim Conservat & Man, Guangdong Entomol Inst, Guangzhou 510260, Guangdong, Peoples R China

[2] Guangdong Prov Wildlife Rescue Ctr, Guangzhou 510520, Guangdong, Peoples R China

来源：

BIOCHEMICAL GENETICS | 2014年 / 52卷 / 3-4期

基金：

中国国家自然科学基金;

关键词：

Big-headed turtle; Platysternon megacephalum; Microsatellite loci; Isolation; Conservation; CONSTRUCTION;

D O I：

10.1007/s10528-014-9640-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The big-headed turtle (Platysternon megacephalum) is critically endangered because of overharvesting, illegal trade, and habitat destruction. Assessment of genetic variability in existing populations becomes very important to the taxonomy and conservation of this species. Here we describe 14 microsatellite loci isolated from an enriched genomic library of the big-headed turtle, and the polymorphisms of these loci were assessed in 28 individuals from Huizhou, Heyuan, Zhaoqing, and Shaoguan of Guangdong, China. The range of polymorphism information content is 0.305-0.738, and no evidence of significant linkage disequilibrium was found among any pairs of loci. These 14 new polymorphic microsatellite loci can be used in population genetics, taxonomy, phylogeography, behavior ecology, and conservation efforts of Platysternon megacephalum.

引用

页码：203 / 208

页数：6

共 16 条

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