B7-1-dependent co-stimulation results in qualitatively and quantitatively different responses by CD4(+) and CD8(+) T cells

被引:74
作者
Deeths, MJ
Mescher, MF
机构
[1] UNIV MINNESOTA,DEPT LAB MED & PATHOL,MINNEAPOLIS,MN 55455
[2] UNIV MINNESOTA,CTR IMMUNOL,MINNEAPOLIS,MN 55455
关键词
B7-1; cytotoxic T lymphocyte; co-stimulation; CD4(+) T cell; CD8(+) T cell;
D O I
10.1002/eji.1830270305
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
To characterize better the co-stimulatory activity of native B7-1 in the absence of other receptor/ligand interactions that might contribute to the response, B7-1 was purified by monoclonal antibody (mAb) affinity chromatography. Immobilization of purified B7-1 with anti-T cell receptor (TCR) mAb on cell-sized latex microspheres provided an effective stimulus for activation of both CD4(+) and CD8(+) T cells as measured by proliferation, development of effector function, and changes in motility and adhesion. The CD4(+) T cell response was prolonged and resulted in efficient interleukin-2 production and clonal expansion. In contrast, CD8(+) responses were transient. Proliferation and clonal expansion peaked on days 3 and 4, coincident with maximal expression of lytic effector function, and the cells then died. These results demonstrate that B7-1 mediated costimulation is sufficient for the induction of effector function in both helper and cytotoxic T cell precursors, but suggest that B7-1 co-stimulation is not sufficient to sustain helper-independent CD8(+) CTL responses. When the dose responses of CD4(+) and CD8(+) T cells to B7-1 were compared, CD8(+) T cells were found to require higher densities of B7-1 to attain an equivalent level of activation, suggesting that the level of expression of B7-1 by APC may influence the development of helper or CTL responses. Finally, in contrast to results obtained by others with B7-1 transfectants, purified B7-1 did not provide co-stimulation when presented on a surface separate from the TCR stimulus.
引用
收藏
页码:598 / 608
页数:11
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