Force Generation by Endocytic Actin Patches in Budding Yeast

被引:49
作者
Carlsson, Anders E. [1 ]
Bayly, Philip V. [2 ]
机构
[1] Washington Univ, Dept Phys, St Louis, MO 63130 USA
[2] Washington Univ, Dept Mech Engn, St Louis, MO 63130 USA
基金
美国国家科学基金会; 美国国家卫生研究院;
关键词
PROPULSION; MECHANICS; PROTEINS; DYNAMICS; MOTILITY; RELEASE; GROWTH;
D O I
10.1016/j.bpj.2014.02.035
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Membrane deformation during endocytosis in yeast is driven by local, templated assembly of a sequence of proteins including polymerized actin and curvature-generating coat proteins such as clathrin. Actin polymerization is required for successful endocytosis, but it is not known by what mechanisms actin polymerization generates the required pulling forces. To address this issue, we develop a simulation method in which the actin network at the protein patch is modeled as an active gel. The deformation of the gel is treated using a finite-element approach. We explore the effects and interplay of three different types of. force driving invagination: 1), forces perpendicular to the membrane, generated by differences between actin polymerization rates at the edge of the patch and those at the center; 2), the inherent curvature of the coat-protein layer; and 3), forces parallel to the membrane that buckle the coat protein layer, generated by an actomyosin contractile ring. We find that with optimistic estimates for the stall stress of actin gel growth and the shear modulus of the actin gel, actin polymerization can generate almost enough force to Overcome the turgor pressure. In combination with the other mechanisms, actin polymerization can the force over the critical value.
引用
收藏
页码:1596 / 1606
页数:11
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