E-cigarettes and Western Diet: Important Metabolic Risk Factors for Hepatic Diseases

被引:35
作者
Hasan, Kamrul M. [1 ]
Friedman, Theodore C. [1 ,2 ]
Shao, Xuesi [1 ,2 ]
Parveen, Meher [1 ]
Sims, Carl [1 ]
Lee, Desean L. [1 ]
Espinoza-Derout, Jorge [1 ]
Sinha-Hikim, Indrani [1 ,2 ]
Sinha-Hikim, Amiya P. [1 ,2 ]
机构
[1] Charles R Drew Univ, Div Endocrinol Metab & Mol Med, Dept Internal Med, Los Angeles, CA USA
[2] Univ Calif Los Angeles, David Geffen Sch Med, Los Angeles, CA 90095 USA
基金
美国国家卫生研究院;
关键词
HIGH-FAT DIET; OXIDATIVE STRESS; INSULIN-RESISTANCE; DEFICIENT MICE; NICOTINE PLUS; STEATOSIS; LIVER; EXPRESSION; AMPK;
D O I
10.1002/hep.30512
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
The use of electronic nicotine delivery systems (ENDS), also known as e-cigarettes, with a variety of e-liquids/e-juices, is increasing at an alarming rate among adolescents who do not realize the potential harmful health effects. This study examines the harmful effects of ENDS on the liver. Apolipoprotein E null (ApoE-/-) mice on a western diet (WD) were exposed to saline or ENDS with 2.4% nicotine aerosol for 12 weeks using our mouse ENDS exposure model system, which delivers nicotine to mice and leads to equivalent serum cotinine levels found in human cigarette users. ApoE-/- mice on a WD exposed to ENDS exhibited a marked increase in hepatic lipid accumulation compared with ApoE-/- on a similar diet exposed to saline aerosol. The detrimental effects of ENDS on hepatic steatosis were associated with significantly greater oxidative stress, increased hepatic triglyceride levels, and increased hepatocyte apoptosis, independent of adenosine monophosphate-activated protein kinase signaling. In addition, hepatic RNA sequencing analysis revealed that 433 genes were differentially expressed in ENDS-exposed mice on WD compared with saline-exposed mice. Functional analysis indicates that genes associated with lipid metabolism, cholesterol biosynthesis, and circadian rhythm were most significantly altered in the liver in response to ENDS. Conclusion: These results demonstrate profound adverse effects of ENDS on the liver. This is important information for regulatory agencies as they regulate ENDS.
引用
收藏
页码:2442 / 2454
页数:13
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