CRISPR-Cas system: Toward a more efficient technology for genome editing and beyond

被引：6
作者：
Ahmadzadeh, Vahideh [1 ]
Farajnia, Safar [1 ,2 ]
Baghban, Roghayyeh [2 ]
Rahbarnia, Leila [3 ]
Zarredar, Habib [4 ]
机构：
[1] Tabriz Univ Med Sci, Drug Appl Res Ctr, Daneshgah Ave, Tabriz, Iran
[2] Tabriz Univ Med Sci, Biotechnol Res Ctr, Tabriz, Iran
[3] Tabriz Univ Med Sci, Infect & Trop Dis Res Ctr, Tabriz, Iran
[4] Tabriz Univ Med Sci, Tuberculosis & Lung Dis Res Ctr, Tabriz, Iran
来源：
JOURNAL OF CELLULAR BIOCHEMISTRY | 2019年 / 120卷 / 10期
关键词：
Genome editing; CRISPR-Cas9; system; Application; Efficiency; KNOCK-IN; TRANSFER-RNA; HUMAN-CELLS; GUIDE-RNA; BASE; DNA; MUTAGENESIS; GENE; GENERATION; PROTEIN;
D O I：
10.1002/jcb.29140
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Genome engineering technology is of great interest for biomedical research that enables scientists to make specific manipulation in the DNA sequence. Early methods for introducing double-stranded DNA breaks relies on protein-based systems. These platforms have enabled fascinating advances, but all are costly and time-consuming to engineer, preventing these from gaining high-throughput applications. The CRISPR-Cas9 system, co-opted from bacteria, has generated considerable excitement in gene targeting. In this review, we describe gene targeting techniques with an emphasis on recent strategies to improve the specificities of CRISPR-Cas systems for nuclease and non-nuclease applications.
引用
收藏
页码：16379 / 16392
页数：14
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