Involvement of histone hypoacetylation in Ni2+-induced bcl-2 down-regulation and human hepatoma cell apoptosis

Although induction of cell apoptosis is known to be involved in the cytotoxicity of Ni2+, little research has been aimed at the mechanism of Ni2+-induced apoptosis. Recent studies showed that Ni2+ induces histone hypoacetylation in different cell lines. Since histone hypoacetylation plays important roles in the control of cell cycle progress and apoptosis, we hypothesized that historic hypoacetylation may be an unrevealed pathway in Ni2+ -induced apoptosis. To address this, effects of Ni2+ on cell apoptosis, bcl-2 gene expression and histone acetylation were examined in human hepatoma Hep3B cells. We found that Ni2+ treatment resulted in cell proliferation arrest, the appearance of detached cells, condensed chromatin, apoptotic bodies and specific DNA fragmentation, indicating the occurrence of cell apoptosis. At the same time, Ni2+ induced a significant decrease in bcl-2 expression and histone acetylation; the decrease of histone H4 acetylation in nucleosomes associated with the bcl-2 promoter region was also proven by a chromatin immunoprecipitation assay, indicating the involvement of histone hypoacetylation in Ni2+-induced bcl-2 downregulation. Further studies showed that increasing histone acetylation by either 100 nM of trichostatin A or over-expressing historic acetyltranferase p300 in Hep3B cells obviously attenuated the bcl-2 down-regulation and cell apoptosis caused by Ni2+. Considering the importance of bcl-2 in determining cell survival and apoptosis, the data presented here suggest that histone hypoacetylation may represent one unrevealed pathway in Ni2+- induced cell apoptosis, where bcl-2 is one of its targets.