Accumulation and detoxification of manganese in hyperaccumulator Phytolacca americana

被引：72

作者：

Dou, C. -M. ^{[1
]}

Fu, X. -P. ^{[1
]}

Chen, X. -C. ^{[1
]}

Shi, J. -Y. ^{[1
]}

Chen, Y. -X. ^{[1
]}

机构：

[1] Zhejiang Univ, Inst Environm Sci & Technol, Hangzhou 310029, Zhejiang, Peoples R China

来源：

PLANT BIOLOGY | 2009年 / 11卷 / 05期

基金：

中国国家自然科学基金;

关键词：

Accumulation; detoxification; manganese; oxalate; Phytolacca americana; MOLECULAR-MECHANISMS; ALUMINUM RESISTANCE; PLANT; TOXICITY; CADMIUM; OXALATE;

D O I：

10.1111/j.1438-8677.2008.00163.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Pokeweed (Phytolacca americana) has recently received much attention because of its ability to hyperaccumulate manganese (Mn). The internal mechanism of detoxification of Mn, however, is not fully understood. In the present study, we investigated Mn accumulation, subcellular distribution, chemical speciation and detoxification through oxalate in pokeweed. The plant accumulated excess Mn in the leaves, mainly in the water-soluble fraction, and over 80% of Mn was in a water-soluble form, while accumulation of excess Mn in the cellular organelle and membrane fraction caused phytotoxicity. In addition, pokeweed has an intrinsically high oxalate content. In all experiments, there was sufficient oxalate to chelate Mn in leaf water extracts at all different levels of Mn application. Phase analysis of X-ray diffraction detected oxalate-Mn chelate complexes, and gel chromatography further confirmed the chelation of Mn by oxalate. In conclusion, pokeweed accumulates excess Mn in the soluble fraction of leaf cells, most likely in vacuoles, in which detoxification of Mn could be achieved by chelation with oxalate.

引用

页码：664 / 670

页数：7

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