Rapid detection of Vibrio cholerae O139 Bengal from stool specimens by PCR

被引：25

作者：

Albert, MJ ^{[1
]}

Islam, D ^{[1
]}

Nahar, S ^{[1
]}

Qadri, F ^{[1
]}

Falklind, S ^{[1
]}

Weintraub, A ^{[1
]}

机构：

[1] HUDDINGE UNIV HOSP,KAROLINSKA INST,DEPT IMMUNOL MICROBIOL PATHOL & INFECT DIS,S-14186 HUDDINGE,SWEDEN

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1997年 / 35卷 / 06期

关键词：

D O I：

10.1128/JCM.35.6.1633-1635.1997

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In a previous study using pure bacterial cultures in a PCR assay, a primer pair corresponding to a unique chromosomal region of Vibrio cholerae O139 Bengal generated an amplicon from only V. cholerae O139 Bengal. PCR with the same primer pair was used to screen 180 diarrheal stool specimens. All the 67 V. cholerae O139 culture-positive stool specimens were positive by PCR, and the remaining specimens, which contained either other recognized enteric pathogens or no pathogens, were all negative by PCR.

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页码：1633 / 1635

页数：3

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