Analysis of gene function in somatic mammalian cells using small interfering RNAs

被引：1068

作者：

Elbashir, SM

Harborth, J

Weber, K

Tuschl, T

机构：

[1] Max Planck Inst Biophys Chem, Dept Biochem & Cell Biol, D-37077 Gottingen, Germany

[2] Max Planck Inst Biophys Chem, Dept Cellular Biochem, D-37077 Gottingen, Germany

来源：

METHODS | 2002年 / 26卷 / 02期

关键词：

RNA interference; small interfering RNA; posttranscriptional gene silencing; knockdown; double-stranded RNA;

D O I：

10.1016/S1046-2023(02)00023-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

RNA interference (RNAi) is a highly conserved gene silencing mechanism that uses double-stranded RNA (dsRNA) as a signal to trigger the degradation of homologous mRNA. The mediators of sequence-specific mRNA degradation are 21- to 23-nt small interfering RNAs (siRNAs) generated by ribonuclease III cleavage from longer dsRNAs. Twenty-one-nuclcotide siRNA duplexes trigger specific gene silencing in mammalian somatic cells without activation of the unspecific interferon response. Here we provide a collection of protocols for siRNA-mediated knockdown of mammalian gene expression. Because of the robustness of the siRNA knockdown technology. genomewide analysis of human gene function in cultured cells has now become possible. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：199 / 213

页数：15

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