Automation of X-ray crystallography

被引：149

作者：

Abola, E

Kuhn, P

Earnest, T

Stevens, RC ^{[1
]}

机构：

[1] Scripps Res Inst, Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA

[2] Syrrx Inc, San Diego, CA 92121 USA

[3] Stanford Univ, Stanford Synchrotron Radiat Lab, Stanford, CA 94305 USA

[4] Univ Calif Berkeley, Lawrence Berkeley Lab, Berkeley Ctr Struct Biol, Phys Biosci Div, Berkeley, CA 94720 USA

[5] Novartis Res Fdn, Genom Inst, San Diego, CA 92121 USA

来源：

NATURE STRUCTURAL BIOLOGY | 2000年 / 7卷 / Suppl 11期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1038/80754

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Structure-based biological discovery is entering a new era with the development of industrialized macromolecular structure determination pipelines. Intense, highly focused X-rays from integrated synchrotron radiation beam lines combined with significant advances in protein expression, purification, and micro-crystallization automation allow for the full streamlining of the traditionally tedious and time consuming process of determining the three dimensional structures of macromolecules.

引用

页码：973 / 977

页数：5

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