Transforming Separation Science with Single-Molecule Methods

被引：14

作者：

Calabrase, William ^{[1
]}

Bishop, Logan D. C. ^{[2
]}

Dutta, Chayan ^{[2
]}

Misiura, Anastasiia ^{[2
]}

Landes, Christy F. ^{[3
,4
]}

Kisley, Lydia ^{[1
,5
]}

机构：

[1] Case Western Reserve Univ, Dept Phys, Cleveland, OH 44106 USA

[2] Rice Univ, Dept Chem, Houston, TX 77251 USA

[3] Rice Univ, Dept Elect & Comp Engn, Dept Chem, Dept Chem & Biomol Engn, Houston, TX 77251 USA

[4] Rice Univ, Smalley Curl Inst, Houston, TX 77251 USA

[5] Case Western Reserve Univ, Dept Chem, Cleveland, OH 44106 USA

来源：

ANALYTICAL CHEMISTRY | 2020年 / 92卷 / 20期

基金：

美国国家科学基金会;

关键词：

FLUORESCENCE CORRELATION SPECTROSCOPY; EXCHANGE ADSORPTION; CHIRAL RECOGNITION; PROTEIN MOLECULES; DNA-MOLECULES; DYNAMICS; RESOLUTION; MICROSCOPY; DIFFUSION; TRACKING;

D O I：

10.1021/acs.analchem.0c02572

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Empirical optimization of the multiscale parameters underlying chromatographic and membrane separations leads to enormous resource waste and production costs. A bottom-up approach to understand the physical phenomena underlying challenges in separations is possible with single-molecule observations of solute-stationary phase interactions. We outline single-molecule fluorescence techniques that can identify key interactions under ambient conditions. Next, we describe how studying increasingly complex samples heightens the relevance of single-molecule results to industrial applications. Finally, we illustrate how separation methods that have not been studied at the single-molecule scale can be advanced, using chiral chromatography as an example case. We hope new research directions based on a molecular approach to separations will emerge based on the ideas, technologies, and open scientific questions presented in this Perspective.

引用

页码：13622 / 13629

页数：8