CD26/DPPIV cell membrane expression and DPPIV activity in plasma of patients with acute leukemia

被引：16

作者：

De Andrade, Camilla F. C. G. ^{[1
]}

Bigni, Ricardo

Pombo-De-Oliveira, Maria S. ^{[2
]}

Alves, Gilda ^{[1
]}

Pereira, Denise A. ^{[1
]}

机构：

[1] Hosp Canc 1, Lab Genet Aplicada, Serv Hematol, Inst Nacl Canc, BR-20230130 Rio De Janeiro, Brazil

[2] Inst Nacl Canc, Div Expt Med, BR-20231050 Rio De Janeiro, Brazil

来源：

JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY | 2009年 / 24卷 / 03期

关键词：

CD26; DPPIV; dipeptidyl peptidase; leukemia; immunophenotype; sitagliptin; inhibition; DIPEPTIDYL-PEPTIDASE-IV; ACUTE LYMPHOBLASTIC-LEUKEMIA; ADENOSINE-DEAMINASE BINDING; AMINO-TERMINAL TRUNCATION; CD26/DIPEPTIDYL-PEPTIDASE IV; FUNCTIONAL-ROLE; LIPID RAFTS; CORD BLOOD; T-CELLS; IN-VIVO;

D O I：

10.1080/14756360802334800

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

CD26/DPPIV (dipeptidil peptidase IV) displays an array of diverse functional properties, with a role in the development of several human cancers. This enzyme is found mainly anchored in the membrane of cells although it also has an enzymatically active plasma isoform. The regulation of biological activities of cytokines by DPP IV activity has a potential role in the homeostatic regulation of hematopoiesis. In this study, we analyzed the CD26 antigen cell membrane expression by flow cytometry and the DPPIV activity in plasma of patients of acute leukemia. The results showed that the plasma DPPIV activity is significantly higher in leukemia patients and could be 100% inhibited by Januvia(TM) (Merck Sharp & Dohme) a selective DPPIV inhibitor. Although CD26 expression on immune cells were not leukemia-dependent the analysis of the correlation between CD26 expression and the DPPIV plasma activity were statistically significant (p < 0.01) in acute lymphoid leukemia (B-ALL and T-ALL).

引用

页码：708 / 714

页数：7

共 35 条

[1] Activation antigen expression on human T cells .1. Analysis by two-colour flow cytometry of umbilical cord blood, adult blood and lymphoid tissue [J].