Proteomic profiling of Ganoderma tsugae ethanol extract-induced adipogenesis displaying browning features

被引：10

作者：

Tseng, Hsiu-Hsueh ^{[1
]}

Yeh, Wan-Chun ^{[2
]}

Tu, Yun-Chen ^{[2
]}

Yang, Bi-Fen ^{[2
]}

Lai, Yen-Ting ^{[2
]}

Lee, Hsien-Kuang ^{[3
]}

Yang, Yo-Chang ^{[2
]}

Huang, Hui-Chi ^{[4
]}

Lee, Yi-Jen ^{[5
]}

Ou, Chien-Chih ^{[6
]}

Kuo, Han-Peng ^{[7
]}

Kuo, Yueh-Hsiung ^{[4
,8
]}

Kao, Ming-Ching ^{[1
,2
]}

Liu, Jah-Yao ^{[1
,9
]}

机构：

[1] Natl Def Med Ctr, Grad Inst Life Sci, Taipei, Taiwan

[2] China Med Univ, Coll Biopharmaceut & Food Sci, Dept Biol Sci & Technol, Taichung, Taiwan

[3] China Med Univ, Coll Med, Grad Inst Basic Med Sci, Taichung, Taiwan

[4] China Med Univ, Coll Chinese Med, Dept Chinese Pharmaceut Sci & Chinese Med Resourc, Taichung, Taiwan

[5] Natl Def Med Ctr, Grad Inst Med Sci, Taipei, Taiwan

[6] Natl Def Med Ctr, Dept Pharmacol, Taipei, Taiwan

[7] Sinphar Grp, Res & Dev Unit, Ilan, Taiwan

[8] China Med Univ, Coll Pharm, Tsuzuki Inst Tradit Med, Taichung, Taiwan

[9] Natl Def Med Ctr, Triserv Gen Hosp, Dept Obstet & Gynecol, Taipei, Taiwan

来源：

FEBS LETTERS | 2018年 / 592卷 / 10期

关键词：

adipogenesis; Ganoderma tsugae; lipid metabolism; metabolic adaptations; proteomics; UCP1; ADIPOSE TRIGLYCERIDE LIPASE; HORMONE-SENSITIVE LIPASE; ADIPOCYTE DIFFERENTIATION; LANOSTANE TRITERPENES; MITOCHONDRIAL BIOGENESIS; LIPID-METABOLISM; FRUITING BODIES; 3T3-L1; CELLS; FAT; TISSUE;

D O I：

10.1002/1873-3468.13061

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ganoderma is classified as a top grade traditional Chinese medicine for promoting human health by regulating 'vital energy'. Its potency towards metabolism and energy homeostasis, particularly, metabolic adaptations of adipocytes, needs to be re-evaluated through an evidence-based study. Here, the triterpenoid-rich Ganoderma tsugae ethanol extract (GTEE) was found to contribute towards adipogenesis accompanied with elevated intracellular lipid metabolic flux. Additionally, proteomic profiling revealed GTEE-upregulated mitochondrial remodeling and chemical energy redox modifications, which display UCP1-positive browning fat-selective features and a NADH-mediated adaptive mechanism. GTEE-treated mice with diet-induced obesity also resulted in the amelioration of white adipocyte hypertrophy and the appearance of UCP1-positive browning adipocytes. Our novel findings unravel that GTEE could promote intracellular metabolic flexibility and plasticity followed by the induction of adipocyte browning.

引用

页码：1643 / 1666

页数：24

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