Discovery of insect and human dengue virus host factors

被引:288
作者
Sessions, October M. [1 ,2 ]
Barrows, Nicholas J. [2 ,3 ,4 ]
Souza-Neto, Jayme A. [7 ,8 ]
Robinson, Timothy J. [1 ,5 ,6 ]
Hershey, Christine L. [9 ]
Rodgers, Mary A. [9 ]
Ramirez, Jose L. [7 ,8 ]
Dimopoulos, George [7 ,8 ]
Yang, Priscilla L. [9 ]
Pearson, James L. [1 ,2 ,3 ]
Garcia-Blanco, Mariano A. [1 ,2 ,3 ,10 ]
机构
[1] Duke Univ, Med Ctr, Dept Mol Genet & Microbiol, Durham, NC 27710 USA
[2] Duke Univ, Med Ctr, Ctr RNA Biol, Durham, NC 27710 USA
[3] Duke Univ, Med Ctr, Duke RNAi Facil, Durham, NC 27710 USA
[4] Duke Univ, Med Ctr, Inst Genome Sci & Policy, Durham, NC 27710 USA
[5] Duke Univ, Med Ctr, Med Scientist Training Program, Durham, NC 27710 USA
[6] Duke Univ, Med Ctr, Program Mol Canc Biol, Durham, NC 27710 USA
[7] Johns Hopkins Univ, Dept Mol Microbiol & Immunol, Baltimore, MD 21205 USA
[8] Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Malaria Res Inst, Baltimore, MD 21205 USA
[9] Harvard Univ, Sch Med, Dept Microbiol & Mol Genet, Boston, MA 02115 USA
[10] Duke NUS Grad Med Sch, Programin Emerging Infect Dis, Singapore 169547, Singapore
基金
美国国家卫生研究院;
关键词
WEST-NILE-VIRUS; ALPHA-GLUCOSIDASE INHIBITORS; JAPANESE ENCEPHALITIS-VIRUS; UNFOLDED PROTEIN RESPONSE; ENDOPLASMIC-RETICULUM; NUCLEAR-LOCALIZATION; ENDOCYTIC PATHWAY; BAFILOMYCIN A1; INFECTED-CELLS; AEDES-AEGYPTI;
D O I
10.1038/nature07967
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Dengue fever is the most frequent arthropod-borne viral disease of humans, with almost half of the world's population at risk of infection(1). The high prevalence, lack of an effective vaccine, and absence of specific treatment conspire to make dengue fever a global public health threat(1,2). Given their compact genomes, dengue viruses (DENV-1-4) and other flaviviruses probably require an extensive number of host factors; however, only a limited number of human, and an even smaller number of insect host factors, have been identified(3-10). Here we identify insect host factors required for DENV-2 propagation, by carrying out a genome-wide RNA interference screen in Drosophila melanogaster cells using a well-established 22,632 double-stranded RNA library. This screen identified 116 candidate dengue virus host factors (DVHFs). Although some were previously associated with flaviviruses (for example, V-ATPases and alpha-glucosidases)(3-5,7,9,10), most of the DVHFs were newly implicated in dengue virus propagation. The dipteran DVHFs had 82 readily recognizable human homologues and, using a targeted short-interfering-RNA screen, we showed that 42 of these are human DVHFs. This indicates notable conservation of required factors between dipteran and human hosts. This work suggests new approaches to control infection in the insect vector and the mammalian host.
引用
收藏
页码:1047 / 1050
页数:4
相关论文
共 30 条
  • [1] Parallels among positive-strand RNA viruses, reverse-transcribing viruses and double-stranded RNA viruses
    Ahlquist, P
    [J]. NATURE REVIEWS MICROBIOLOGY, 2006, 4 (05) : 371 - 382
  • [2] Effect of bafilomycin A1 on the growth of Japanese encephalitis virus in Vero cells
    Andoh, T
    Kawamata, H
    Umatake, M
    Terasawa, K
    Takegami, T
    Ochiai, H
    [J]. JOURNAL OF NEUROVIROLOGY, 1998, 4 (06) : 627 - 631
  • [3] Genome-wide RNAi analysis of growth and viability in Drosophila cells
    Boutros, M
    Kiger, AA
    Armknecht, S
    Kerr, K
    Hild, M
    Koch, B
    Haas, SA
    Paro, R
    Perrimon, N
    [J]. SCIENCE, 2004, 303 (5659) : 832 - 835
  • [4] Antiviral effect of α-glucosidase inhibitors on viral morphogenesis and binding properties of hepatitis C virus-like particles
    Chapel, C
    Garcia, C
    Roingeard, P
    Zitzmann, N
    Dubuisson, J
    Dwek, RA
    Trépo, C
    Zoulim, F
    Durantel, D
    [J]. JOURNAL OF GENERAL VIROLOGY, 2006, 87 : 861 - 871
  • [5] Infectious entry of West Nile virus occurs through a clathrin-mediated endocytic pathway
    Chu, JJH
    Ng, ML
    [J]. JOURNAL OF VIROLOGY, 2004, 78 (19) : 10543 - 10555
  • [6] α-glucosidase inhibitors reduce dengue virus production by affecting the initial steps of virion morphogenesis in the endoplasmic reticulum
    Courageot, MP
    Frenkiel, MP
    Santos, CDD
    Deubel, V
    Desprès, P
    [J]. JOURNAL OF VIROLOGY, 2000, 74 (01) : 564 - 572
  • [7] Anopheles gambiae immune responses to human and rodent Plasmodium parasite species
    Dong, Yuemei
    Aguilar, Ruth
    Xi, Zhiyong
    Warr, Emma
    Mongin, Emmanuel
    Dimopoulos, George
    [J]. PLOS PATHOGENS, 2006, 2 (06) : 513 - 525
  • [8] Interaction of TIA-1/TIAR with West Nile and dengue virus products in infected cells interferes with stress granule formation and processing body assembly
    Emara, Mohamed M.
    Brinton, Margo A.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (21) : 9041 - 9046
  • [9] Engineering RNA interference-based resistance to dengue virus type 2 in genetically modified Aedes aegypti
    Franz, AWE
    Sanchez-Vargas, I
    Adelman, ZN
    Blair, CD
    Beaty, BJ
    James, AA
    Olson, KE
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (11) : 4198 - 4203
  • [10] STRUCTURAL-CHANGES AND FUNCTIONAL CONTROL OF THE TICK-BORNE ENCEPHALITIS-VIRUS GLYCOPROTEIN-E BY THE HETERODIMERIC ASSOCIATION WITH PROTEIN PRM
    HEINZ, FX
    STIASNY, K
    PUSCHNERAUER, G
    HOLZMANN, H
    ALLISON, SL
    MANDL, CW
    KUNZ, C
    [J]. VIROLOGY, 1994, 198 (01) : 109 - 117