Beneficial effects of splenectomy on massive hepatectomy model in rats

被引：29

作者：

Arakawa, Yusuke ^{[1
]}

Shimada, Mitsuo ^{[1
]}

Uchiyama, Hideaki ^{[1
]}

Ikegami, Toru ^{[1
]}

Yoshizumi, Tomoharu ^{[1
]}

Imura, Satoru ^{[1
]}

Morine, Yuji ^{[1
]}

Kanemura, Hirohumi ^{[1
]}

机构：

[1] Univ Tokushima, Inst Hlth Biosci, Dept Surg, Grad Sch, Tokushima 7708503, Japan

来源：

HEPATOLOGY RESEARCH | 2009年 / 39卷 / 04期

关键词：

heat shock protein; hemeoxygenase-1; hepatectomy; liver regeneration; reperfusion injury; splenectomy; AMELIORATES HEPATIC ISCHEMIA; MICROSOMAL HEME OXYGENASE; SPLENIC ARTERY LIGATION; GROWTH-FACTOR ACTIVITY; LIVER-TRANSPLANTATION; HEPATOCELLULAR-CARCINOMA; REPERFUSION INJURY; BLOOD-FLOW; REGENERATION; HYPERSPLENISM;

D O I：

10.1111/j.1872-034X.2008.00469.x

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Possible spleno-hepatic relationships during hepatectomy remain unclear. The purpose of this study was to investigate the impact of splenectomy during massive hepatectomy in rats. Rats were divided into the following two groups: 90% hepatectomy (Hx group), hepatectomy with splenectomy (Hx+Sp group). The following parameters were evaluated; survival rate, biochemical parameters, quantitative RT-PCR for hemeoxygenase-1 (HO-1) and tumor necrosing factor alpha (TNF alpha), immunohistochemical staining for HO-1, proliferating cell nuclear antigen labeling index and liver weights. The survival rate after massive hepatectomy significantly improved in Hx+Sp group as well as serum biochemical parameters, compared with Hx group (P < 0.05). HO-1 positive hepatocytes and its mRNA expression significantly increased and TNF alpha mRNA expression significantly decreased in Hx+Sp group compared with Hx group (P < 0.05). Moreover, liver regeneration was significantly accelerated at 48 and 72 h after hepatectomy in Hx+Sp group. Splenectomy had beneficial effects on massive hepatectomy by ameliorating liver injuries and promoting preferable liver regeneration.

引用

页码：391 / 397

页数：7

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