Surface plasmon resonance imaging of Au nano-partifcle modified DNA monolayers

被引：0
作者：
Yamaguchi, A [1 ]
Hayashida, M [1 ]
Juodkazis, S [1 ]
Hara, Y [1 ]
Matsuo, S [1 ]
Misawa, H [1 ]
机构：
[1] Univ Tokushima, Satellite Venture Business Lab Photon Nano Mat, Tokushima 7708506, Japan
来源：
BIOMEDICAL NANOTECHNOLOGY ARCHITECTURES AND APPLICATIONS | 2002年 / 4626卷
关键词：
DNA; hybridization; SPR; Au nano-particle; AFM;
D O I：
10.1117/12.491216
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A probe DNA monolayer modified with Au nano-particles was prepared on an Au thin film and used for DNA hybridization detection by surface plasmon resonance (SPR) spectroscopy. This Au particle modified probe DNA monolayer resulted in about a 3.5 times larger SPR angle shift according to the hybridization reaction, compared to that found in a non-modified probe DNA monolayer. By comparison of the SPR image of the particle modified and non-modified probe DNA monolayer, this modification method was shown to be useful in improving the SPR signal for the detection of the unlabelled DNA molecules.
引用
收藏
页码：247 / 251
页数：5
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