A Rapid Cytologic Double Staining of Epstein-Barr Virus-encoded Small RNA and Cell Surface Markers for Diagnosis of Epstein-Barr Virus-associated Hemophagocytic Lymphohistiocytosis

被引：1

作者：

Takahashi, Natsuki ^{[1
]}

Kudo, Ko ^{[1
]}

Tanaka, Miyuki ^{[3
]}

Kumagai, Naoya ^{[2
]}

Sato, Tomohiko ^{[1
]}

Kamio, Takuya ^{[1
]}

Sasaki, Shinya ^{[1
]}

Terui, Kiminori ^{[1
]}

Kurose, Akira ^{[2
]}

Yanagisawa, Ryu ^{[3
,4
]}

Nakazawa, Yozo ^{[3
]}

Ito, Etsuro ^{[1
]}

机构：

[1] Hirosaki Univ, Grad Sch Med, Dept Pediat, 5 Zaifucho, Hirosaki, Aomori 0368562, Japan

[2] Hirosaki Univ, Grad Sch Med, Dept Anat Pathol, Hirosaki, Aomori, Japan

[3] Shinshu Univ, Sch Med, Dept Pediat, Matsumoto, Nagano, Japan

[4] Shinshu Univ Hosp, Div Blood Transfus, Matsumoto, Nagano, Japan

来源：

JOURNAL OF PEDIATRIC HEMATOLOGY ONCOLOGY | 2020年 / 42卷 / 08期

基金：

日本学术振兴会;

关键词：

hemophagocytic lymphohistiocytosis; Epstein-Barr virus; Epstein-Barr virus– encoded small RNA; cytologic diagnosis; ETOPOSIDE;

D O I：

10.1097/MPH.0000000000001647

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A 3-year-old boy was clinically diagnosed with Epstein-Barr virus (EBV)-associated hemophagocytic lymphohistiocytosis. We identified EBV-infected CD8-positive T-lymphocytes by cytologic double staining of the peripheral blood for EBV-encoded small RNA and cell surface markers. The patient was subsequently administered immunosuppressive therapy with a reduced dose of etoposide because of previous organ damage. EBV clearance was confirmed by serial quantification of cell-fractionated EBV-DNA, whereas EBV-DNA persisted in the plasma for 18 weeks. Immunochemotherapy with low-dose etoposide combined with serial viral load monitoring is a potential therapeutic option for severe EBV-hemophagocytic lymphohistiocytosis cases with organ damage.

引用

页码：E756 / E758

页数：3

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