Micro-Raman Analysis of Sperm Cells on Glass Slide: Potential Label-Free Assessment of Sperm DNA toward Clinical Applications

被引:5
作者
Du, Shengrong [1 ,2 ]
Zhang, Qun [3 ]
Guan, Haohao [3 ]
Chen, Guannan [3 ]
Wang, Sisi [4 ]
Sun, Yan [2 ]
Li, Yuling [3 ]
Chen, Rong [3 ]
He, Youwu [3 ]
Huang, Zufang [3 ]
机构
[1] Fujian Normal Univ, Coll Life Sci, Biomed Res Ctr South China, Key Lab Innate Immune Biol Fujian Prov, Fuzhou 350117, Peoples R China
[2] Fujian Matern & Child Hlth Hosp, Reprod Ctr, Fuzhou 350001, Peoples R China
[3] Fujian Normal Univ, Key Lab Optoelect Sci & Technol Med, Fujian Prov Key Lab Photon Technol, Minist Educ, Fuzhou 350007, Peoples R China
[4] Fujian Med Univ, Dept Gen Surg, Union Hosp, Fuzhou 350001, Peoples R China
来源
BIOSENSORS-BASEL | 2022年 / 12卷 / 11期
基金
中国国家自然科学基金;
关键词
Raman spectroscopy; sperm DNA; multivariate statistical analysis; MULTIPLICATIVE SIGNAL CORRECTION; SPECTROSCOPY; SUBTRACTION; DAMAGE; FRAGMENTATION; INTEGRITY; SUBSTRATE; CHOICE; BLOOD;
D O I
10.3390/bios12111051
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Routine assessment of sperm DNA integrity involves the time-consuming and complex process of staining sperm chromatin. Here, we report a Raman spectroscopy method combined with extended multiplicative signal correction (EMSC) for the extraction of characteristic fingerprints of DNA-intact and DNA-damaged sperm cells directly on glass slides. Raman results of sperm cell DNA integrity on glass substrates were validated one-to-one with clinical sperm cell staining. Although the overall Raman spectral pattern showed considerable similarity between DNA-damaged and DNA-intact sperm cells, differences in specific Raman spectral responses were observed. We then employed and compared multivariate statistical analysis based on principal component analysis-linear discriminant analysis (PCA-LDA) and partial least-squares-discriminant analysis (PLS-DA), and the classifications were validated by leave-one-out-cross-validation (LOOCV) and k-fold cross-validation methods. In comparison, the PLS-DA model showed relatively better results in terms of diagnostic sensitivity, specificity, and the classification rate between the sperm DNA damaged group and the DNA intact group. Our results demonstrate the potential of Raman based label-free DNA assessment of sperm cell on glass substrates as a simple method toward clinical applications.
引用
收藏
页数:11
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