ANALYSIS OF A SINGLE BIOMOLECULE TRANSITING WITH NANOPORE

With the development of science and technology, much more aspects of nano-technology have been used for studying macrobiomolecules, but seldom for single-molecular detection (SMD). We reviews recent results in the research of DNA molecules with nanopores, introduce basic concept and technology which can be used to study the disassembling dynamics of nucleosomes. We present here that the two transport parameters of blockade current and duration yield the sequence and the structural information of the collagen-like peptides and a-helix peptides. As the nanopore is the only channel the conducts ions, when the large biomolecules is being translocated through the pore under the applied electric field, a passing ion current blockade will be observed, which is based on the relationship of the protein's conformation feature with the change of ion current parameters. Nanopore analysis clearly showed the blockade current and duration is, related to the tertiary structures of peptides. The new method could be possible to develop a highly sensitive analysis for the conformation change of protein related to human disease.