Network analysis of human Y microsatellite haplotypes

To investigate the utility of Y chromosome microsatellites for studying human male-lineage evolution, we typed samples from three populations for five tetranucleotide repeats and an Alu insertion polymorphism, We found very high levels of haplotype diversity and evidence that most mutations involve the gain or loss of only one repeat unit, implying that any given microsatellite haplotype may have arisen independently on two or more Y-chromosome lineages, Together, these factors suggest that interpretation of small sample sizes (<30) will be problematic, By typing a large sample of individuals (n = 174) from one population, East Anglia, we were able to construct a haplotype network, The network exhibits a well-connected core structure of commoner haplotypes, Computer simulations based on this network estimate the convergence time for African and Caucasian groups may be between 1.4 and 1.8 times as long as the convergence of the East Anglian population, Based on our comparison between large and small sample sizes, we suggest that large sample sizes are necessary in order to interpret Y-microsatellite haplotypes, and that a network analysis of the type we describe may prove informative in future studies.