Monodisperse Cell-Encapsulating Peptide Microgel Beads for 3D Cell Culture

被引：89

作者：

Tsuda, Yukiko ^{[1
,2
]}

Morimoto, Yuya ^{[1
]}

Takeuchi, Shoji ^{[1
,2
]}

机构：

[1] Univ Tokyo, Inst Ind Sci, Meguro Ku, Tokyo 1538505, Japan

[2] Life BEANS Ctr, BEANS Project, Meguro Ku, Tokyo 1538505, Japan

来源：

LANGMUIR | 2010年 / 26卷 / 04期

关键词：

SCAFFOLDS; OLIGOPEPTIDE; SYSTEM;

D O I：

10.1021/la902827y

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

This paper describes a method to produce monodisperse cell-encapsulating microgel beads composed of a self-assembling peptide gel for three-dimensional (3D) cell culture. We used a 3D microfluidic axisymmetric now-focusing device with ail external gelation method. The finely powdered salts were dispersed into a continuous phase, and the Salts induced the gelation when in contact with the peptide solution. Over 93% of the cells survived after the encapsulation, and (he cells migrated and grew Within the gels. Applications of Our cell-encapsulating beads include bead-based cell assays in drug testing and engineering tissue constructs.

引用

页码：2645 / 2649

页数：5

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