Fast and efficient separation of immunoglobulin M from immunoglobulin G using short monolithic columns

被引：28

作者：

Brne, P. ^{[1
]}

Podgornik, A. ^{[1
]}

Bencina, K. ^{[1
]}

Gabor, B. ^{[1
]}

Strancar, A. ^{[1
]}

Peterka, M. ^{[1
]}

机构：

[1] BIA Separat Doo, SI-1000 Ljubljana, Slovenia

来源：

JOURNAL OF CHROMATOGRAPHY A | 2007年 / 1144卷 / 01期

关键词：

CIM monoliths; IgM; IgG; chromatography;

D O I：

10.1016/j.chroma.2006.12.044

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Certain diagnostic, analytical and preparative applications require the separation of immunoglobulin G (IgG) from immunoglobulin M (IgM). In the present work, different ion-exchange methacrylate monoliths were tested for the separation of IgG and IgM. The strong anion-exchange column had the highest dynamic binding capacity reaching more than 20 mg of IgM/ml of support. Additionally, separation of IgM from human serum albumin, a common contaminant in immunoglobulin purification, was achieved on the weak ethylenediamino anion-exchange column, which set the basis for the IgM purification method developed on convective interaction media (CIM) supports. Experiments also confirmed flow independent characteristics of the short monolithic columns. (c) 2006 Elsevier B.V All rights reserved.

引用

页码：120 / 125

页数：6

共 15 条

[1]

[Anonymous], IMMUNOCHEMISTRY

[2] EVALUATION OF A SIMPLIFIED SUCROSE GRADIENT METHOD FOR DETECTION OF RUBELLA-SPECIFIC IGM IN ROUTINE DIAGNOSTIC PRACTICE [J].