Metaproteomics approach to study the functionality of the microbiota in the human infant gastrointestinal tract

被引：125

作者：

Klaassens, Eline S. ^{[1
]}

de Vos, Willem M. ^{[1
]}

Vaughan, Elaine E. ^{[1
]}

机构：

[1] Univ Wageningen & Res Ctr, Microbiol Lab, NL-6703 CT Wageningen, Netherlands

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2007年 / 73卷 / 04期

关键词：

D O I：

10.1128/AEM.01921-06

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A metaproteomics approach comprising two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization-time of flight (mass spectrometry) was applied to the largely uncultured infant fecal microbiota for the first time. The fecal microbial metaproteome profiles changed over time, and one protein spot contained a peptide sequence that showed high similarity to those of bifidobacterial transaidolases.

引用

页码：1388 / 1392

页数：5

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