Optimization and mass culture of Acutodesmus obliquus RDS01 under open phototrophic pond cultivation for enhancing biodiesel production

In the optimization of medium for open phototrophic pond cultivation of microalgae, Acutodesmus obliquus RDS01, response surface methodology (RSM) was used and lipid, biomass and biodiesel production were analyzed. The experimental investigation showed that a good achievement and regression coefficient was determined highly (R-2 = 0.999) in analysis of variance (ANOVA). The effect of six different macro-elements (NaNO3, KH2 PO4, K2HPO4, MgSO 4, CaCl2 and NaCl) on growth of Acutodesmus obliquus RDS01 was premeditated in bold basal medium (BBM). The absolute concentration of lipid was 220.07 mg mL(-1), biomass 14.49 g L-1 and biodiesel 3.9 mL g(-1). The results achieved in phototrophic open pond cultivation using optimized medium were approximately 2 times higher than in the control medium. Fourier transform infrared (FTIR) analysis confirmed the presence of a lipid profile functional group similar to biodiesel and major fatty acid methyl ester (FAME) profiling viz., tetradecanoic acid (C14: 0), hexadecanoic acid (C16: 0), octadecanoic acid (C18: 0), (Z)-Octadec-9-enoic acid (C18: 1), linoleic acid (C18: 2), alpha-linolenic acid (C18: 3), cis-11-eicosenoic acid (C20: 5), 13-cis-docosenoic acid (C22: 6) on gas chromatography and mass spectrometric (GC-MS) analysis. A high productivity of lipid, biomass and biodiesel from isolated freshwater microalgae was found.