Macrophage-secreted factors impair human adipogenesis: Involvement of proinflammatory state in preadipocytes

被引:248
|
作者
Lacasa, Daniele
Taleb, Soraya
Keophiphath, Mayoura
Miranville, Alexandra
Clement, Karine
机构
[1] Hop Hotel Dieu, Serv Nutr, INSERM, Unite 755,EA 3502, F-75004 Paris, France
[2] Univ Paris 06, Fac Med Les Cordeliers, F-75004 Paris, France
[3] Hop Hotel Dieu, AP HP, Dept Nutr, F-75004 Paris, France
[4] Goethe Univ Frankfurt, Cardiovasc Physiol Inst, D-60385 Frankfurt, Germany
关键词
D O I
10.1210/en.2006-0687
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Obesity is considered a chronic low-grade inflammatory state. The white adipose tissue produces a variety of inflammation-related proteins whose expression is increased in obese subjects. The nonadipose cell fraction, which includes infiltrated macrophages, is a determinant source of inflammation-related molecules within the adipose tissue. Our working hypothesis is that macrophage infiltration affects fat expansion through a paracrine action on adipocyte differentiation. Human primary preadipocytes were then differentiated in the presence of conditioned media obtained from macrophages differentiated from blood monocytes. Preadipocytes treated by macrophage-conditioned medium displayed marked reduction of adipogenesis as assessed by decreased cellular lipid accumulation and reduced gene expression of adipogenic and lipogenic markers. In addition to this effect, the activation of macrophages by lipopolysaccharides stimulated nuclear factor kappa B signaling, increased gene expression and release of proinflammatory cytokines and chemokines, and induced preadipocyte proliferation. This phenomenon was associated with increased cyclin D1 gene expression and maintenance of the fibronectin-rich matrix. Anti-TNF alpha neutralizing antibody inhibits the inflammatory state of preadipocytes positioning TNF alpha as an important mediator of inflammation in preadipocytes. Strikingly, conditioned media produced by macrophages isolated from human adipose tissue exerted comparable effects with activated macrophages, i.e. decreased adipogenesis and increased inflammatory state in the preadipocytes. These data show that macrophage-secreted factors inhibit the formation of mature adipocytes, suggesting possible role in limiting adipose tissue expansion in humans.
引用
收藏
页码:868 / 877
页数:10
相关论文
共 32 条
  • [1] Macrophage-Secreted Factors Promote a Profibrotic Phenotype in Human Preadipocytes
    Keophiphath, Mayoura
    Achard, Vincent
    Henegar, Corneliu
    Rouault, Christine
    Clement, Karine
    Lacasa, Daniele
    MOLECULAR ENDOCRINOLOGY, 2009, 23 (01) : 11 - 24
  • [2] Macrophage-secreted factors inhibit ZAG expression and secretion by human adipocytes
    Gao, D.
    Trayhurn, P.
    Bing, C.
    MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2010, 325 (1-2) : 135 - 142
  • [3] Macrophage-secreted factors induce adipocyte inflammation and insulin resistance
    Permana, PA
    Menge, C
    Reaven, PD
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2006, 341 (02) : 507 - 514
  • [4] Macrophage-secreted Factors that Rejuvenate Delayed Fracture Healing Characteristic of Aging
    Sekar, M.
    Alman, B. A.
    JOURNAL OF THE AMERICAN GERIATRICS SOCIETY, 2015, 63 : S149 - S149
  • [5] Selection of multipotent cells and enhanced muscle reconstruction by myogenic macrophage-secreted factors
    Malerba, Alberto
    Vitiello, Libero
    Segat, Daniela
    Dazzo, Emanuela
    Frigo, Marco
    Scambi, Ilaria
    De Coppi, Paolo
    Boldrin, Luisa
    Martelli, Laura
    Pasut, Alessandro
    Romuoldi, Chiara
    Bellomo, Rosa Grazia
    Vecchiet, Jacopo
    Baroni, Mourizio David
    EXPERIMENTAL CELL RESEARCH, 2009, 315 (06) : 915 - 927
  • [6] REGULATORY EFFECTS OF MACROPHAGE-SECRETED FACTORS ON LYMPHOCYTE-T COLONY GROWTH
    SREDNI, B
    MICHLIN, H
    KALECHMAN, I
    ROZENSZAJN, LA
    ISRAEL JOURNAL OF MEDICAL SCIENCES, 1979, 15 (02): : 193 - 193
  • [7] REGULATORY EFFECTS OF MACROPHAGE-SECRETED FACTORS ON T-LYMPHOCYTE COLONY GROWTH
    SREDNI, B
    MICHLIN, H
    KALECHMAN, Y
    ROZENSZAJN, LA
    CELLULAR IMMUNOLOGY, 1978, 36 (01) : 15 - 27
  • [8] Endometriotic Epithelial Cell Response to Macrophage-Secreted Factors is Dependent on Extracellular Matrix Context
    Pollock, Kathryn
    Jaraczewski, Taylor J.
    Carroll, Molly J.
    Lebovic, Dan I.
    Kreeger, Pamela K.
    CELLULAR AND MOLECULAR BIOENGINEERING, 2014, 7 (03) : 409 - 420
  • [9] Gremlin-1, a Secreted BMP Antagonist by Human Preadipocytes, Modifies Beige Adipogenesis
    Gustafson, Birgit
    Salari, Hamid
    Grimsby, Joseph
    Rondinone, Cristina
    Smith, Ulf
    DIABETES, 2014, 63 : A511 - A511
  • [10] Endometriotic Epithelial Cell Response to Macrophage-Secreted Factors is Dependent on Extracellular Matrix Context
    Kathryn Pollock
    Taylor J. Jaraczewski
    Molly J. Carroll
    Dan I. Lebovic
    Pamela K. Kreeger
    Cellular and Molecular Bioengineering, 2014, 7 : 409 - 420