The purification and characterisation of allergenic hazelnut seed proteins

被引：38

作者：

Rigby, Neil M. ^{[1
]}

Marsh, Justin ^{[2
]}

Sancho, Ana I. ^{[1
]}

Wellner, Klaus ^{[1
]}

Akkerdaas, Jaap ^{[3
]}

van Ree, Ronald ^{[3
]}

Knulst, Andre ^{[4
]}

Fernandez-Rivas, Montserrat ^{[5
]}

Brettlova, Vlasta ^{[6
]}

Schilte, Piet P. ^{[7
]}

Summer, Colin ^{[8
]}

Pumphrey, Richard ^{[8
]}

Shewry, Peter R. ^{[2
]}

Mills, E. N. Clare ^{[1
]}

机构：

[1] Inst Food Res, Norwich NR4 7UA, Norfolk, England

[2] Rothamsted Res, Harpenden, Herts, England

[3] Univ Amsterdam, Acad Med Ctr, Dept Expt Immunol, NL-1012 WX Amsterdam, Netherlands

[4] UMC Utrecht, Dept Dermatol & Allergol, Utrecht, Netherlands

[5] Hosp Clin San Carlos, Madrid, Spain

[6] Prinsengracht Ziekenhuis, Dept Allergol, Amsterdam, Netherlands

[7] Med Ctr Alkmaar, Dept Pediat, Alkmaar, Netherlands

[8] Manchester Royal Infirm, Dept Immunol, Allergy Res Grp, Manchester M13 9WL, Lancs, England

来源：

MOLECULAR NUTRITION & FOOD RESEARCH | 2008年 / 52卷

基金：

英国生物技术与生命科学研究理事会;

关键词：

Allergens; Characterisation; EuroPrevall; Hazelnut; Purification;

D O I：

10.1002/mnfr.200800083

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A lipid transfer protein (LTP, Cor a 8) together with the 11S (Cor a 9) and 7S seed storage globulins (Cor a 11) are major food allergens present in hazelnut. Methods are described for their purification and characterisation using in-gel tryptic digestion mass spectrometry to confirm their identities and circular dichroism and Fourier-transform infrared spectroscopies to demonstrate that they are authentically folded. Preliminary immunochemical studies have also confirmed that the purified preparations retain their immunological properties in terms of immunoglobulin E binding, determined by immunoblotting using serum from hazelnut allergic patients. These preparations form a basis for development of improved methods of diagnosis of food allergy based on the concept of component-resolved diagnosis.

引用

页码：S251 / S261

页数：11

共 21 条

[1] Enhancement of hazelnut extract for IgE testing by recombinant allergen spiking [J].