CpG oligodeoxynucleotides directly induce CXCR3 chemokines in human B cells

被引：23

作者：

Kato, A

Ogasawara, T

Homma, T

Batchelor, J

Imai, S

Wakiguchi, H

Saito, H

Matsumoto, K

机构：

[1] Natl Res Inst Child Hlth & Dev, Dept Allergy & Immunol, Setagaya Ku, Tokyo 1548567, Japan

[2] Nikken Chem Co Ltd, Pharmaceut Res Labs, Omiya, Saitama 3300835, Japan

[3] Kochi Med Sch, Dept Mol Microbiol & Infect, Nankoku, Kochi 7838505, Japan

[4] Kochi Med Sch, Dept Pediat, Nankoku, Kochi 7838505, Japan

[5] RIKEN Res Ctr Allergy & Immunol, Res Unit Allergy Transcriptome, Tsurumi Ku, Yokohama, Kanagawa 2300045, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 320卷 / 04期

关键词：

CpG ODN; CXCR3; chemokines; IP-10; Mig; I-TAC; B cells; TLR9;

D O I：

10.1016/j.bbrc.2004.06.059

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

CpG oligodeoxynucleotides (CpG ODN) are known to elicit Th1 immune responses via TLR9. However, the precise mechanisms through which B cells are involved in this phenomenon are not fully understood. We investigated the effect of CpG ODN on the induction of Th1-chemoattractant CXCR3 chemokines, IP-10, Mig, and I-TAC, in B cells. Cells from the RPMI 8226 human B cell line and human peripheral B cells were stimulated with three distinct classes of CpG ODN. As a result, CXCR3 chemokines were strongly up-regulated by CpG-B and CpG-C, but only weakly by CpG-A. Though CXCR3 chemokines are known to be induced by IFNs, blocking mAbs against IFN receptors did not inhibit their induction by CpG-B. Induction of CXCR3 chemokines was blocked by two NF-kappaB inhibitors and a p38 inhibitor. These results strongly suggest that CXCR3 chemokines are directly induced by CpG ODN via NF-kappaB- and p38-dependent pathways in human B cells. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：1139 / 1147

页数：9

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