Isolation and Characterization of Adipose-derived Mesenchymal Stem Cells (ADSCs) from Cattle

被引：35

作者：

Lu, Taofeng ^{[1
,2
]}

Xiong, Hui ^{[3
]}

Wang, Kunfu ^{[1
]}

Wang, Shuo ^{[1
]}

Ma, Yuehui ^{[1
]}

Guan, Weijun ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China

[2] Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Harbin 150001, Peoples R China

[3] Jiamusi Univ, Coll Med, Jiamusi 154007, Peoples R China

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2014年 / 174卷 / 02期

关键词：

Bovine; Adipose-derived mesenchymal stem cells; Differentiation; MARROW STROMAL CELLS; BONE-MARROW; MYOGENIC DIFFERENTIATION; TRANSDIFFERENTIATION; CHICKEN; SYSTEM; REPAIR; TISSUE;

D O I：

10.1007/s12010-014-1128-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Adipose-derived mesenchymal stem cells (ADSCs) were isolated from the adult adipose tissue of 2-year-old cattle, and then characterized by immunofluorescence and RT-PCR. We found that primary bADSCs could be expanded for 25 passages. Expression of beta-integrin, CD44, and CD73 was observed by immunofluorescence and RT-PCR. Passage 3 bADSCs were successfully induced to differentiate into osteoblasts and adipocytes. The results indicate the potential for multi-lineage differentiation of bADSCs that may represent an ideal candidate for cellular transplantation therapy.

引用

页码：719 / 728

页数：10

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