The Cytotoxicity of ZnO NPs on Breast Cancer Cell Lines MCF-7 Depending on the Dispersion Solution

However, the number of in vitro studies are being published regarding the cytotoxicity of nanomaterials is on the increase, the components of the media for toxicity assays have often varied according to the needs of the scientists. Our aim for this study was to: (1) evaluate the influence of serum-in this case, fetal bovine serum FBS, Phosphate Buffer Saline PBS, water for injection in a cell culture medium on the toxicity of nano-sized (20-30 nm) ZnO on human breast cancer cell line (MCF-7) and (2) use very small dose that make 50% of cancer cell death after treatment in order to the normal cells can get rid of it and be safe on body normal cells. Dispersion solution is effective factor of cell viability in presence of ZnO NPs as a treatment. The nano sized ZnO exhibited their highest toxicity when exposed to Phosphate buffer saline, in contrast to exposure in media containing 10% serum. This mainly comes from the absence of protein protection, and lower cell growth rate, but also that when we used sterilized water for injection as the dispersion solution it has an observed toxicity and a negative osmotic effect on cells that make cells increase till exploding.