MULTIPLEX SYBR® GREEN-REAL TIME PCR (qPCR) ASSAY FOR THE DETECTION AND DIFFERENTIATION OF Bartonella henselae AND Bartonella clarridgeiae IN CATS

被引：23

作者：

Staggemeier, Rodrigo ^{[1
]}

Pilger, Diogo Andre ^{[2
]}

Spilki, Fernando Rosado ^{[1
]}

Cantarelli, Vlademir Vicente ^{[1
,3
]}

机构：

[1] Univ Feevale, Mol Microbiol Lab, BR-93352000 Novo Hamburgo, RS, Brazil

[2] Univ Fed Rio Grande do Sul, Fac Farm, BR-90046900 Porto Alegre, RS, Brazil

[3] Lab Qualita, Novo Hamburgo, RS, Brazil

来源：

REVISTA DO INSTITUTO DE MEDICINA TROPICAL DE SAO PAULO | 2014年 / 56卷 / 02期

关键词：

Bartonella; Cat Scratch Disease; qPCR; COMB-NOV; IDENTIFICATION; SPP; PREVALENCE; PROPOSALS; UNIFY;

D O I：

10.1590/S0036-46652014000200001

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

A novel SYBR (R) green-real time polymerase chain reaction (qPCR) was developed to detect two Bartonella species, B. henselae and B. clarridgeiae, directly from blood samples. The test was used in blood samples obtained from cats living in animal shelters in Southern Brazil. Results were compared with those obtained by conventional PCR targeting Bartonella spp. Among the 47 samples analyzed, eight were positive using the conventional PCR and 12 were positive using qPCR. Importantly, the new qPCR detected the presence of both B. henselae and B. clarridgeiae in two samples. The results show that the qPCR described here may be a reliable tool for the screening and differentiation of two important Bartonella species.

引用

页码：93 / 95

页数：3

共 24 条

[1] Bartonella spp. as emerging human pathogens [J].

Anderson, BE ;

Neuman, MA .

CLINICAL MICROBIOLOGY REVIEWS, 1997, 10 (02) :203-+

[2]

ANGULO FJ, 1994, J AM VET MED ASSOC, V205, P1711

[3] Prevalence of Bartonella species in domestic cats in the Netherlands [J].

Bergmans, AMC ;

deJong, CMA ;

vanAmerongen, G ;

Schot, CS ;

Schouls, LM .

JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (09) :2256-2261

[4] Vector transmission of Bartonella species with emphasis on the potential for tick transmission [J].

Billeter, S. A. ;

Levy, M. G. ;

Chomel, B. B. ;

Breitschwerdt, E. B. .

MEDICAL AND VETERINARY ENTOMOLOGY, 2008, 22 (01) :1-15

[5] DIFFERENTIATION OF BARTONELLA SPECIES USING RESTRICTION-ENDONUCLEASE ANALYSIS OF PCR-AMPLIFIED 16S RIBOSOMAL-RNA GENES [J].

BIRTLES, RJ .

FEMS MICROBIOLOGY LETTERS, 1995, 129 (2-3) :261-265

[6] PROPOSALS TO UNIFY THE GENERA GRAHAMELLA AND BARTONELLA, WITH DESCRIPTIONS OF BARTONELLA-TALPAE COMB-NOV, BARTONELLA-PEROMYSCI COMB-NOV, AND 3 NEW SPECIES, BARTONELLA-GRAHAMII SP-NOV, BARTONELLA-TAYLORII SP-NOV, AND BARTONELLA-DOSHIAE SP-NOV [J].

BIRTLES, RJ ;

HARRISON, TG ;

SAUNDERS, NA ;

MOLYNEUX, DH .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1995, 45 (01) :1-8

[7] Factors associated with the rapid emergence of zoonotic Bartonella infections [J].

Boulouis, HJ ;

Chang, CC ;

Henn, JB ;

Kasten, RW ;

Chomel, BB .

VETERINARY RESEARCH, 2005, 36 (03) :383-410

[8] PROPOSALS TO UNIFY THE GENERA BARTONELLA AND ROCHALIMAEA, WITH DESCRIPTIONS OF BARTONELLA-QUINTANA COMB-NOV, BARTONELLA-VINSONII COMB-NOV, BARTONELLA-HENSELAE COMB-NOV, AND BARTONELLA-ELIZABETHAE COMB-NOV, AND TO REMOVE THE FAMILY BARTONELLACEAE FROM THE ORDER RICKETTSIALES [J].

BRENNER, DJ ;

OCONNOR, SP ;

WINKLER, HH ;

STEIGERWALT, AG .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1993, 43 (04) :777-786

[9] Evaluation of Immunohistochemistry in Identifying Bartonella henselae in Cat-Scratch Disease [J].

Caponetti, Gabriel C. ;

Pantanowitz, Liron ;

Marconi, Sharon ;

Havens, Jennifer M. ;

Lamps, Laura W. ;

Otis, Christopher N. .

AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 2009, 131 (02) :250-256

[10] Molecular evidence of Bartonella spp. in questing adult Ixodes pacificus ticks in California [J].

Chang, CC ;

Chomel, BB ;

Kasten, RW ;

Romano, V ;

Tietze, N .

JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (04) :1221-1226

← 1 2 3 →