Effects of Abscisic Acid and Salicylic Acid on Gene Expression in the Antiviral RNA Silencing Pathway in Arabidopsis

被引:43
作者
Alazem, Mazen [1 ,2 ,3 ]
Kim, Kook-Hyung [1 ,2 ,4 ]
Lin, Na-Sheng [3 ]
机构
[1] Seoul Natl Univ, Coll Agr & Life Sci, Dept Agr Biotechnol, Seoul 08826, South Korea
[2] Seoul Natl Univ, Coll Agr & Life Sci, Plant Genom & Breeding Inst, Seoul 08826, South Korea
[3] Acad Sinica, Inst Plant & Microbial Biol, Taipei 11529, Taiwan
[4] Seoul Natl Univ, Coll Agr & Life Sci, Res Inst Agr & Life Sci, Seoul 08826, South Korea
基金
新加坡国家研究基金会;
关键词
abscisic acid; salicylic acid; crosstalk; RNA silencing pathway; bamboo mosaic virus; ACTIVATED TRANSCRIPTION FACTOR; DNA-BINDING PROTEINS; BAMBOO-MOSAIC-VIRUS; REGULATORY NETWORKS; FUNCTIONAL-ANALYSIS; DISEASE RESISTANCE; SEED-GERMINATION; DOMAIN PROTEINS; PLANT HORMONES; BASAL DEFENSE;
D O I
10.3390/ijms20102538
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The RNA silencing pathways modulate responses to certain stresses, and can be partially tuned by several hormones such as salicylic acid (SA) and abscisic acid (ABA). Although SA and ABA are often antagonistic and often modulate different stress responses, they have similar effects on virus resistance, which are partially achieved through the antiviral RNA silencing pathway. Whether they play similar roles in regulating the RNA silencing pathway is unclear. By employing coexpression and promoter analyses, we found that some ABA- and SA-related transcription factors (TFs) are coexpressed with several AGO, DCL, and RDR genes, and have multiple binding sites for the identified TFs in the queried promoters. ABA and SA are antagonistic with respect to the expression of AGO1 and RDRs because ABA was able to induce these genes only in the SA mutant. Nevertheless, both hormones showed similarities in the regulation of other genes, for example, the induction of AGO2 by ABA was SA-dependent, indicating that ABA acts upstream of SA in this regulation. We inferred that the similar effects of ABA and SA on some genes resulted in the redundancy of their roles in resistance to bamboo mosaic virus, but that the two hormones are antagonistic with respect to other genes unrelated to their biosynthesis pathways.
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页数:16
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