Expression of sphingosine kinase gene in the interactions between human gastric carcinoma cell and vascular endothelial cell

被引:5
作者
Ren, J [1 ]
Dong, L
Xu, CB
Pan, BR
机构
[1] Xi An Jiao Tong Univ, Hosp 1, Dept Oncol Radiotherapy, Xian 710061, Shaanxi, Peoples R China
[2] Xi An Jiao Tong Univ, Hosp 2, Dept Gastroenterol, Xian 710004, Peoples R China
[3] Lund Univ, Dept Pathophysiol, Lund, Sweden
[4] Fourth Mil Med Univ, Xijing Hosp, Ctr Oncol, Xian 710032, Peoples R China
关键词
D O I
10.3748/wjg.v8.i4.602
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
AIM: To study the interactions between human gastric carcinoma cell (HGCC) and human vascular endothelial cell (HVEC), and if the expression of sphingosine kinase (SPK) gene was involved in these interactions. METHODS: The specific inhibitor to SPK, dimethyl sphingosine (DMS), was added acting on HGCC and HVEC, then the cell proliferation was measured by MTT. The conditioned mediums (CMs) of HGCC and HVEC were prepared. The CM of one kind of cell was added to the other kind of cell, and the cell proliferation was measured by MTT. After the action of CM, the cellular expression of SPK gene in mRNA level was detected with in situ hybridization (ISH). RESULTS: DMS could almost completely inhibit the proliferation of HGCC and HVEC. The growth inhibitory rates could amount to 97.21%, 83.42%, respectively (P<0.01). The CM of HGCC could stimulate the growth of HVEC (2.70 +/- 0.01, P<0.01) while the CM of HVEC could inhibit the growth of HGCC (52.97 +/- 0.01%, P<0.01). There was no significant change in the mRNA level of SPK gene in one kind of cell after the action of the CM of the other kind of cell. CONCLUSION: SPK plays a key role in regulating the proliferation of HGCC and HVEC. There exist complicated interactions between HGCC and HVEC. HGCC can significantly stimulate the growth of HVEC while HVEC can significantly inhibit the growth of HGCC. The expression of SPK gene is not involved in the interactions.
引用
收藏
页码:602 / 607
页数:6
相关论文
共 49 条
  • [1] Inhibition of angiogenesis and vascular tumor growth by interferon-producing cells -: A gene therapy approach
    Albini, A
    Marchisone, C
    Del Grosso, F
    Benelli, R
    Masiello, L
    Tacchetti, C
    Bono, M
    Ferrantini, M
    Rozera, C
    Truini, M
    Belardelli, F
    Santi, L
    Noonan, DM
    [J]. AMERICAN JOURNAL OF PATHOLOGY, 2000, 156 (04) : 1381 - 1393
  • [2] Depolarisation induces rapid and transient formation of intracellular sphingosine-1-phosphate
    Alemany, R
    Kleuser, B
    Ruwisch, L
    Danneberg, K
    Lass, H
    Hashemi, R
    Spiegel, S
    Jakobs, KH
    Heringdorf, DMZ
    [J]. FEBS LETTERS, 2001, 509 (02) : 239 - 244
  • [3] Extracellular export of sphingosine kinase-1 enzyme - Sphingosine 1-phosphate generation and the induction of angiogenic vascular maturation
    Ancellin, N
    Colmont, C
    Su, J
    Li, Q
    Mittereder, N
    Chae, SS
    Stefansson, S
    Liau, G
    Hla, T
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (08) : 6667 - 6675
  • [4] Effects of vascular endothelial growth factor and E-selectin on angiogenesis in the murine metastatic RCT sarcoma
    Aoki, M
    Kanamori, M
    Yudoh, K
    Ohmori, K
    Yasuda, T
    Kimura, T
    [J]. TUMOR BIOLOGY, 2001, 22 (04) : 239 - 246
  • [5] VEGF is upregulated in a neuroblastoma and hepatocyte coculture model
    Beierle, EA
    Strande, LF
    Berger, AC
    Chen, MK
    [J]. JOURNAL OF SURGICAL RESEARCH, 2001, 97 (01) : 34 - 40
  • [6] Migration of vascular smooth muscle cells induced by sphingosine 1-phosphate and related lipids: Potential role in the angiogenic response
    Boguslawski, G
    Grogg, JR
    Welch, Z
    Ciechanowicz, S
    Sliva, D
    Kovala, AT
    McGlynn, P
    Brindley, DN
    Rhoades, RA
    English, D
    [J]. EXPERIMENTAL CELL RESEARCH, 2002, 274 (02) : 264 - 274
  • [7] Angiogenesis is an early event in the generation of myc-induced lymphomas
    Brandvold, KA
    Neiman, P
    Ruddell, A
    [J]. ONCOGENE, 2000, 19 (23) : 2780 - 2785
  • [8] Chronic effects of fumonisin B1 in broilers and turkeys fed dietary treatments to market age
    Broomhead, JN
    Ledoux, DR
    Bermudez, AJ
    Rottinghaus, GE
    [J]. POULTRY SCIENCE, 2002, 81 (01) : 56 - 61
  • [9] Cai L, 2001, WORLD J GASTROENTERO, V7, P506
  • [10] Distinct effects of different calcium-mobilizing agents on cell death in NG108-15 neuroblastoma X glioma cells
    Chin, TY
    Hwang, HM
    Chueh, SH
    [J]. MOLECULAR PHARMACOLOGY, 2002, 61 (03) : 486 - 494