Proteome Dynamics and Physiological Responses to Short-Term Salt Stress in Brassica napus Leaves

被引:32
作者
Jia, Huan [1 ]
Shao, Mingquan [1 ]
He, Yongjun [1 ]
Guan, Rongzhan [1 ,2 ]
Chu, Pu [1 ]
Jiang, Haidong [1 ]
机构
[1] Nanjing Agr Univ, Coll Agr, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing, Jiangsu, Peoples R China
[2] Jiangsu Collaborat Innovat Ctr Modern Crop Prod, Nanjing, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
CINNAMYL ALCOHOL-DEHYDROGENASE; QUANTITATIVE PROTEOMICS; ARABIDOPSIS-THALIANA; PLANT; PHOTOSYNTHESIS; EXPRESSION; PROTEINS; SALINITY; WHEAT; MECHANISMS;
D O I
10.1371/journal.pone.0144808
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Salt stress limits plant growth and crop productivity and is an increasing threat to agriculture worldwide. In this study, proteomic and physiological responses of Brassica napus leaves under salt stress were investigated. Seedlings under salt treatment showed growth inhibition and photosynthesis reduction. A comparative proteomic analysis of seedling leaves exposed to 200 mM NaCl for 24 h, 48 h and 72 h was conducted. Forty-four protein spots were differentially accumulated upon NaCl treatment and 42 of them were identified, including several novel salt-responsive proteins. To determine the functional roles of these proteins in salt adaptation, their dynamic changes in abundance were analyzed. The results suggested that the up-accumulated proteins, which were associated with protein metabolism, damage repair and defense response, might contribute to the alleviation of the deleterious effect of salt stress on chlorophyll biosynthesis, photosynthesis, energy synthesis and respiration in Brassica napus leaves. This study will lead to a better understanding of the molecular basis of salt stress adaptation in Brassica napus and provides a basis for genetic engineering of plants with improved salt tolerance in the future.
引用
收藏
页数:21
相关论文
共 70 条
[31]  
Manaa A, 2014, COMPARATIVE PROTEOMI, V15, P21803
[32]   ATP synthase of chloroplast thylakoid membranes: A more in depth characterization of its ATPase activity [J].
McCarty, RE .
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES, 2005, 37 (05) :289-297
[33]   Responses of photosynthesis, chlorophyll fluorescence and ROS-Scavenging systems to salt stress during seedling and reproductive stages in rice [J].
Moradi, Foad ;
Ismail, Abdelbagi M. .
ANNALS OF BOTANY, 2007, 99 (06) :1161-1173
[34]   Approaches to increasing the salt tolerance of wheat and other cereals [J].
Munns, R ;
James, RA ;
Läuchli, A .
JOURNAL OF EXPERIMENTAL BOTANY, 2006, 57 (05) :1025-1043
[35]   An accurate and reproducible method for proteome profiling of the effects of salt stress in the rice leaf lamina [J].
Parker, R ;
Flowers, TJ ;
Moore, AL ;
Harpham, NVJ .
JOURNAL OF EXPERIMENTAL BOTANY, 2006, 57 (05) :1109-1118
[36]   Salt-stress induced changes in the leaf proteome of diploid and tetraploid mandarins with contrasting Na+ and Cl- accumulation behaviour [J].
Podda, Alessandra ;
Checcucci, Giovanni ;
Mouhaya, Wafa ;
Centeno, Delphine ;
Rofidal, Valerie ;
Del Carratore, Renata ;
Luro, Francois ;
Morillon, Raphael ;
Ollitrault, Patrick ;
Maserti, Bianca Elena .
JOURNAL OF PLANT PHYSIOLOGY, 2013, 170 (12) :1101-1112
[37]   Towards salinity tolerance in Brassica: An overview [J].
Purty R.S. ;
Kumar G. ;
Singla-Pareek S.L. ;
Pareek A. .
Physiology and Molecular Biology of Plants, 2008, 14 (1-2) :39-49
[38]   Proteo mics-based dissection of stress-responsive pathways in plants [J].
Qureshi, M. Irfan ;
Qadir, S. ;
Zolla, Lello .
JOURNAL OF PLANT PHYSIOLOGY, 2007, 164 (10) :1239-1260
[39]   Comparative proteomics unveils cross species variations in Anabaena under salt stress [J].
Rai, Snigdha ;
Agrawal, Chhavi ;
Shrivastava, Alok Kumar ;
Singh, Prashant Kumar ;
Rai, L. C. .
JOURNAL OF PROTEOMICS, 2014, 98 :254-270
[40]   Proteorne analysis of tobacco leaves under salt stress [J].
Razavizadeh, Roya ;
Ehsanpour, Ali Akbar ;
Ahsan, Nagib ;
Komatsu, Setsuko .
PEPTIDES, 2009, 30 (09) :1651-1659