High-resolution NMR-based metabolic detection of microgram biopsies using a 1 mm HRμMAS probe

被引：14

作者：

Nishiyama, Yusuke ^{[1
,2
]}

Endo, Yuki ^{[1
]}

Nemoto, Takahiro ^{[1
]}

Bouzier-Sore, Anne-Karine ^{[3
]}

Wong, Alan ^{[4
]}

机构：

[1] JEOL RESONANCE Inc, Akishima, Tokyo 1968558, Japan

[2] RIKEN CLST JEOL Collaborat Ctr, Yokohama, Kanagawa 2300045, Japan

[3] CNRS Univ Bordeaux, Ctr Resonance Magnet Syst Biol, UMR5536, Bordeaux, France

[4] CEA Saclay, DSM, IRAMIS, CEA CNRS NIMBE UMR3685,Lab Struct & Dynam Resona, F-91191 Gif Sur Yvette, France

来源：

ANALYST | 2015年 / 140卷 / 24期

关键词：

NUCLEAR-MAGNETIC-RESONANCE; HIGH-SENSITIVITY; IN-VIVO; SPECTROSCOPY; STRATEGY;

D O I：

10.1039/c5an01810b

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A prototype 1 mm High-Resolution micro-Magic Angle Spinning (HR mu MAS) probe is described. High quality H-1 NMR spectra were obtained from 490 mu g of heterogeneous biospecimens, offering a rich-metabolite profiling. The results demonstrate the potential of HR mu MAS as a new NMR analytical tool in metabolomics.

引用

页码：8097 / 8100

页数：4

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