Novel self-association fibronectin sites

被引：9

作者：

Carnemolla, B ^{[1
]}

Leprini, A ^{[1
]}

Querze, G ^{[1
]}

Urbini, S ^{[1
]}

Zardi, L ^{[1
]}

机构：

[1] IST NAZL RIC CANC,CELL BIOL LAB,I-16132 GENOA,ITALY

来源：

BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE | 1996年 / 74卷 / 06期

关键词：

fibronectin; type III repeats interaction;

D O I：

10.1139/o96-081

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, we report a strong interaction between two contiguous proteolytic fragments of fibronectin, each having a mass of about 16 kDa. This interaction was stable in 4 M NaCl and 4 M urea and dissociation of the two fragments required buffers containing 0.5% sodium dodecyl sulphate. After purification, these peptides maintained their ability to interact when mixed. One fragment was made up of type III repeat 4 and part of 5, the other by repeat 6 and part of 5. Such strong interaction between two fibronectin regions may play a role in fibronectin conformation as well as during fibronectin fibril formation.

引用

收藏

页码：745 / 748

页数：4

相关论文

共 12 条

[1] PERICELLULAR MATRIX IN MALIGNANT TRANSFORMATION [J].

ALITALO, K ;

VAHERI, A .

ADVANCES IN CANCER RESEARCH, 1982, 37 :111-158

[2] LARGE-SCALE PROCEDURE FOR THE PURIFICATION OF FIBRONECTIN DOMAINS [J].

BORSI, L ;

CASTELLANI, P ;

BALZA, E ;

SIRI, A ;

PELLECCHIA, C ;

DESCALZI, F ;

ZARDI, L .

ANALYTICAL BIOCHEMISTRY, 1986, 155 (02) :335-345

[3]

CARNEMOLLA B, 1992, J BIOL CHEM, V267, P24689

[4]

Hynes R. O., 1990, FIBRONECTINS

[5] PRIMARY STRUCTURE OF HUMAN FIBRONECTIN - DIFFERENTIAL SPLICING MAY GENERATE AT LEAST 10 POLYPEPTIDES FROM A SINGLE GENE [J].

KORNBLIHTT, AR ;

UMEZAWA, K ;

VIBEPEDERSEN, K ;

BARALLE, FE .

EMBO JOURNAL, 1985, 4 (07) :1755-1759

[6] STRUCTURE OF A FIBRONECTIN TYPE-III DOMAIN FROM TENASCIN PHASED BY MAD ANALYSIS OF THE SELENOMETHIONYL PROTEIN [J].

LEAHY, DJ ;

HENDRICKSON, WA ;

AUKHIL, I ;

ERICKSON, HP .

SCIENCE, 1992, 258 (5084) :987-991

[7] DOMAIN-STRUCTURE AND INTERACTIONS OF THE TYPE-I AND TYPE-II MODULES IN THE GELATIN-BINDING REGION OF FIBRONECTIN - ALL 6 MODULES ARE INDEPENDENTLY FOLDED [J].

LITVINOVICH, SV ;

STRICKLAND, DK ;

MEDVED, LV ;

INGHAM, KC .

JOURNAL OF MOLECULAR BIOLOGY, 1991, 217 (03) :563-575

[8] THE 3-DIMENSIONAL STRUCTURE OF THE 10TH TYPE-III MODULE OF FIBRONECTIN - AN INSIGHT INTO RGD-MEDIATED INTERACTIONS [J].

MAIN, AL ;

HARVEY, TS ;

BARON, M ;

BOYD, J ;

CAMPBELL, ID .

CELL, 1992, 71 (04) :671-678

[9]

Mosher, 1989, FIBRONECTIN, P1

[10] LOCATION OF THE CELL-ATTACHMENT SITE IN FIBRONECTIN WITH MONOCLONAL-ANTIBODIES AND PROTEOLYTIC FRAGMENTS OF THE MOLECULE [J].

PIERSCHBACHER, MD ;

HAYMAN, EG ;

RUOSLAHTI, E .

CELL, 1981, 26 (02) :259-267