Mining wild barley for powdery mildew resistance

被引:32
作者
Ames, N. [1 ]
Dreiseitl, A. [2 ]
Steffenson, B. J. [3 ]
Muehlbauer, G. J. [1 ,4 ]
机构
[1] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA
[2] Agrotest Fyto Ltd, Dept Integrated Plant Protect, Kromeriz 76701, Czech Republic
[3] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA
[4] Univ Minnesota, Dept Plant Biol, St Paul, MN 55108 USA
关键词
Blumeria graminis f. sp hordei; genome-wide association study; Hordeum vulgare subsp spontaneum; powdery mildew; wild barley; DISEASE RESISTANCE; GENES; POPULATION; DIVERSITY; SOFTWARE; HORDEI; LOCUS; LINES; RUST; MAP;
D O I
10.1111/ppa.12384
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Powdery mildew, caused by Blumeria graminis f. sp. hordei (Bgh), is a worldwide disease problem on barley (Hordeum vulgare) with potentially severe impact on yield. Historically, resistance genes have been identified chiefly from cultivated lines and landraces; however, wild barley (H. vulgare subsp. spontaneum) accessions have proven to be extraordinarily rich sources of powdery mildew resistance. This study describes the characterization of a collection of 316 wild barley accessions, known as the Wild Barley Diversity Collection (WBDC), for resistance to powdery mildew and the genetic location of powdery mildew resistance loci. The WBDC was phenotyped for reaction to 40 different Bgh isolates at the seedling stage and genotyped with 10 508 molecular markers. Accessions resistant to all 40 isolates of Bgh were not found; however, three accessions (WBDC 053, 085 and 089) exhibited resistance to 38 of the isolates. Gene postulation analyses revealed that many accessions, while resistant, contained none of the 12 genes present in the Pallas near-isogenic lines Mla1, Mla3, Mla6, Mla7, Mla9, Mla12, Mla13, Mlk1, MlLa, Mlg, Mlat and Ml(Ru2), suggesting that the accessions carry novel genes or gene combinations. A genome-wide association study of powdery mildew resistance in the WBDC identified 21 significant marker-trait associations that resolved into 15 quantitative trait loci. Seven of these loci have not been previously associated with powdery mildew resistance. Taken together, these results demonstrate that the WBDC is a rich source of powdery mildew resistance, and provide genetic tools for incorporating the resistance into barley breeding programmes.
引用
收藏
页码:1396 / 1406
页数:11
相关论文
共 41 条
[1]   Basal Host Resistance of Barley to Powdery Mildew: Connecting Quantitative Trait Loci and Candidate Genes [J].
Aghnoum, Reza ;
Marcel, Thierry C. ;
Johrde, Annika ;
Pecchioni, Nicola ;
Schweizer, Patrick ;
Niks, Rients E. .
MOLECULAR PLANT-MICROBE INTERACTIONS, 2010, 23 (01) :91-102
[2]   Development of wild barley-derived DArT markers and their integration into a barley consensus map [J].
Alsop, B. P. ;
Farre, A. ;
Wenzl, P. ;
Wang, J. M. ;
Zhou, M. X. ;
Romagosa, I. ;
Kilian, A. ;
Steffenson, B. J. .
MOLECULAR BREEDING, 2011, 27 (01) :77-92
[3]   Signaling in plant-microbe interactions [J].
Baker, B ;
Zambryski, P ;
Staskawicz, B ;
DineshKumar, SP .
SCIENCE, 1997, 276 (5313) :726-733
[4]  
Barrett Jeffrey C, 2009, Cold Spring Harb Protoc, V2009, DOI 10.1101/pdb.ip71
[5]   TASSEL: software for association mapping of complex traits in diverse samples [J].
Bradbury, Peter J. ;
Zhang, Zhiwu ;
Kroon, Dallas E. ;
Casstevens, Terry M. ;
Ramdoss, Yogesh ;
Buckler, Edward S. .
BIOINFORMATICS, 2007, 23 (19) :2633-2635
[6]   FACTORS AFFECTING BUILDUP OF FUNGICIDE RESISTANCE IN POWDERY MILDEW IN SPRING BARLEY [J].
BRENT, KJ ;
CARTER, GA ;
HOLLOMON, DW ;
HUNTER, T ;
LOCKE, T ;
PROVEN, M .
NETHERLANDS JOURNAL OF PLANT PATHOLOGY, 1989, 95 :31-41
[7]   Fitness costs and trade-offs of disease resistance and their consequences for breeding arable crops [J].
Brown, J. K. M. ;
Rant, J. C. .
PLANT PATHOLOGY, 2013, 62 :83-95
[8]   The barley mlo gene: A novel control element of plant pathogen resistance [J].
Buschges, R ;
Hollricher, K ;
Panstruga, R ;
Simons, G ;
Wolter, M ;
Frijters, A ;
vanDaelen, R ;
vanderLee, T ;
Diergaarde, P ;
Groenendijk, J ;
Topsch, S ;
Vos, P ;
Salamini, F ;
Schulze-Lefert, P .
CELL, 1997, 88 (05) :695-705
[9]   Development and implementation of high-throughput SNP genotyping in barley [J].
Close, Timothy J. ;
Bhat, Prasanna R. ;
Lonardi, Stefano ;
Wu, Yonghui ;
Rostoks, Nils ;
Ramsay, Luke ;
Druka, Arnis ;
Stein, Nils ;
Svensson, Jan T. ;
Wanamaker, Steve ;
Bozdag, Serdar ;
Roose, Mikeal L. ;
Moscou, Matthew J. ;
Chao, Shiaoman ;
Varshney, Rajeev K. ;
Szuecs, Peter ;
Sato, Kazuhiro ;
Hayes, Patrick M. ;
Matthews, David E. ;
Kleinhofs, Andris ;
Muehlbauer, Gary J. ;
DeYoung, Joseph ;
Marshall, David F. ;
Madishetty, Kavitha ;
Fenton, Raymond D. ;
Condamine, Pascal ;
Graner, Andreas ;
Waugh, Robbie .
BMC GENOMICS, 2009, 10
[10]   Development of DArT Marker Platforms and Genetic Diversity Assessment of the US Collection of the New Oilseed Crop Lesquerella and Related Species [J].
Cruz, Von Mark V. ;
Kilian, Andrzej ;
Dierig, David A. .
PLOS ONE, 2013, 8 (05)