γ-H2AX foci are increased in lymphocytes in vivo in young children 1 h after very low-dose X-irradiation: a pilot study

被引:36
作者
Halm, Brunhild M. [1 ]
Franke, Adrian A. [1 ]
Lai, Jennifer F. [1 ]
Turner, Helen C. [2 ]
Brenner, David J. [2 ]
Zohrabian, Vatche M. [2 ]
DiMauro, Robert [3 ]
机构
[1] Univ Hawaii, Ctr Canc, Honolulu, HI 96813 USA
[2] Columbia Univ, Ctr Radiol Res, Med Ctr, New York, NY 10032 USA
[3] Kapiolani Med Ctr Women & Children, Honolulu, HI 96826 USA
关键词
Children; Radiation dose; Lymphocytic gamma-H2AX foci formation; Computed tomography; DOUBLE-STRAND BREAKS; COMPUTED-TOMOGRAPHY; LOW-LEVEL; IONIZING-RADIATION; DNA-DAMAGE; UNITED-STATES; HISTONE H2AX; CANCER-RISKS; HELICAL CT; EXPOSURE;
D O I
10.1007/s00247-014-2983-3
中图分类号
R72 [儿科学];
学科分类号
100202 ;
摘要
Computed tomography (CT) is an imaging modality involving ionizing radiation. The presence of gamma-H2AX foci after low to moderate ionizing radiation exposure has been demonstrated; however it is unknown whether very low ionizing radiation exposure doses from CT exams can induce gamma-H2AX formation in vivo in young children. To test whether very low ionizing radiation doses from CT exams can induce lymphocytic gamma-H2AX foci (phosphorylated histones used as a marker of DNA damage) formation in vivo in young children. Parents of participating children signed a consent form. Blood samples from three children (ages 3-21 months) undergoing CT exams involving very low blood ionizing radiation exposure doses (blood doses of 0.22-1.22 mGy) were collected immediately before and 1 h post CT exams. Isolated lymphocytes were quantified for gamma-H2AX foci by a technician blinded to the radiation status and dose of the patients. Paired t-tests and regression analyses were performed with significance levels set at P < 0.05. We observed a dose-dependent increase in gamma-H2AX foci post-CT exams (P = 0.046) among the three children. Ionizing radiation exposure doses led to a linear increase of foci per cell in post-CT samples (102% between lowest and highest dose). We found a significant induction of gamma-H2AX foci in lymphocytes from post-CT samples of three very young children. When possible, CT exams should be limited or avoided by possibly applying non-ionizing radiation exposure techniques such as US or MRI.
引用
收藏
页码:1310 / 1317
页数:8
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