Enzymatic Synthesis of Structured Triacylglycerols Containing CLA Isomers Starting from sn-1,3-Diacylglycerols

被引:25
作者
Maurelli, Silvia [1 ]
Blasi, Francesca [1 ]
Cossignani, Lina [1 ]
Bosi, Ancilla [1 ]
Simonetti, Maria Stella [1 ]
Damiani, Pietro [1 ]
机构
[1] Univ Perugia, Dipartimento Sci Econ Estimat & Alimenti, Sez Chim Bromatol, I-06126 Perugia, Italy
关键词
CLA; sn-1,3-DAG; Lipozyme IM; Structured lipids; CONJUGATED LINOLEIC-ACID; OIL; ATHEROSCLEROSIS; ACIDOLYSIS; METABOLISM; TAG;
D O I
10.1007/s11746-008-1334-7
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
The synthesis of structured triacylglycerols (TAG) by the enzymatic reaction between sn-1,3-diacylglycerols (sn-1,3-DAG) and conjugated linoleic acid (CLA) isomers was studied. Both the substrates of the reaction were produced from vegetable oils, the sn-1,3-DAG from extra virgin olive oil and the CLA isomers from sunflower oil. The enzymatic reactions between these substrates were catalyzed for 96 h by an immobilized lipase from Rhizomucor miehei (Lipozyme IM) and the reactions carried out in solvent were monitored every 24 h by using high-performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD). The enzymatic reactions were carried out in different reaction media (hexane, isooctane and solvent free) and with different CLA/sn-1,3-DAG ratios. Total % acidic composition and structural analysis data were evaluated to verify the presence of CLA isomers in sn-2- position of synthesized TAG. The results showed good levels of CLA incorporation in sn-1,3-DAG, from 19.2% of TAG synthesized in solvent free conditions with a 0.5:1 substrate ratio, to 47.5% of TAG synthesized in isooctane with a 2:1 substrate ratio. It was observed that for all the reaction media, the best sn-2- acylic specificity was obtained with a 0.5:1 substrate ratio.
引用
收藏
页码:127 / 133
页数:7
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