Ring-opening polymerization of e-caprolactone catalyzed by a novel lipase Candida sp 99-125

被引：6

作者：

He, Wei ^{[1
]}

Fang, Zheng ^{[2
]}

Zhu, Ning ^{[1
]}

Ji, Dong ^{[1
]}

Li, Zhenjiang ^{[1
]}

Guo, Kai ^{[1
]}

机构：

[1] Nanjing Univ Technol, Coll Biotechnol & Pharmaceut Engn, State Key Lab Mat Oriented Chem Engn, 30 Puzhu Rd S, Nanjing 211816, Jiangsu, Peoples R China

[2] Nanjing Univ Technol, Sch Pharmaceut Sci, Nanjing 211816, Jiangsu, Peoples R China

来源：

BIOCATALYSIS AND BIOTRANSFORMATION | 2015年 / 33卷 / 03期

关键词：

ring-opening polymerization; epsilon-caprolactone; enzymatic catalysis; Candida sp; 99-125; ALIPHATIC POLYESTERS; LACTONES; POLY(EPSILON-CAPROLACTONE); POLYCAPROLACTONE; MORPHOLOGY; POLYMERS; SOLVENT; ESTERS; BLENDS; ROUTE;

D O I：

10.3109/10242422.2015.1089864

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ring-opening polymerization of -caprolactone catalyzed by a novel lipase Candida sp. 99-125 was achieved in organic solvents. -Caprolactone was found to be polymerized in quantitative yield in toluene at 80 degrees C for 5 days. The poly (-caprolactone) obtained had a number-average molecular weight of 14,120 (polydispersity index=1.161). The effect of enzyme concentration, temperature, reaction time, reaction medium, and water content on monomer conversion and product molecular weight were investigated. Kinetic analysis showed that the enzyme displayed Michaelis-Menten kinetics and showed stronger affinity for -caprolactone (with a K-m value of 6.1mmol/L) than other reported lipases.

引用

页码：150 / 155

页数：6

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