Germination as a process to increase the polyphenol content and antioxidant activity of lupin seeds (Lupinus angustifolius L.)

被引:173
作者
Duenas, M. [1 ]
Hernandez, T. [2 ]
Estrella, I. [2 ]
Fernandez, D. [2 ]
机构
[1] Univ Salamanca, Grp Invest Polifenoles, Unidad Nutr & Bromatol, Fac Farm, Salamanca 37007, Spain
[2] CSIC, Inst Fermentac Ind, E-28006 Madrid, Spain
关键词
Lupin seed; Germination; Polyphenols; Flavonoids; Antioxidant activity; PHENOLIC-COMPOUNDS; EXOGENOUS ENZYMES; PISUM-SATIVUM; FLAVONOIDS; COTYLEDON; CAPACITY; COAT; VARIETIES; LENTILS; LEGUMES;
D O I
10.1016/j.foodchem.2009.04.051
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
In this work, the effect of germination of lupin seeds (Lupinus angustifolius L., c.v. Zapaton) on bioactive phenolic compounds as well as on the antioxidant activity was studied. Phenolic compounds were analysed by HPLC-PAD-ESI/MS. The antioxidant activity was determined by spectrophotometry, evaluating the free radical scavenging activity of the samples. Germination produced significant changes in flavonoids and non-flavonoid phenolic compounds. In the analysed samples, isoflavones, flavones and dihydroflavonols in free and conjugated forms were identified. The results obtained indicate that germination modifies the quantitative and qualitative polyphenolic composition of lupin (Lupinus angustifolius L.) seeds during the different days of the process, with a significant increase of flavonoids. An increase in the antioxidant activity was also observed as a consequence of the process. Germination was shown to be a good process to increase the phenolic content of lupin seeds as well as their antioxidant activity. (c) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码:599 / 607
页数:9
相关论文
共 38 条
[1]  
AMAROWICZ R, 2008, EUROPEAN J LIPID SCI, V110, P856
[2]   Antioxidant activity of extract of adzuki bean and its fractions [J].
Amarowicz, Ryszard ;
Estrella, Isabel ;
Hernandez, Teresa ;
Troszynska, Agnieszka .
JOURNAL OF FOOD LIPIDS, 2008, 15 (01) :119-136
[3]   Antioxidant activities of isoflavones and their biological metabolites in a liposomal system [J].
Arora, A ;
Nair, MG ;
Strasburg, GM .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1998, 356 (02) :133-141
[4]   Isolation and characterization of novel benzoates, cinnamates, flavonoids, and lignans from Riesling wine and screening for antioxidant activity [J].
Baderschneider, B ;
Winterhalter, P .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2001, 49 (06) :2788-2798
[5]   Profiling of flavonoid conjugates in Lupinus albus and Lupinus angustifolius responding to biotic and abiotic stimuli [J].
Bednarek, P ;
Kerhoas, L ;
Einhorn, J ;
Franski, R ;
Wojtaszek, P ;
Rybus-Zajac, M ;
Stobiecki, M .
JOURNAL OF CHEMICAL ECOLOGY, 2003, 29 (05) :1127-1142
[6]  
BRAND-WILLIAMS W, 1995, FOOD SCI TECHNOL-LEB, V28, P25
[7]   High-performance liquid chromatography determination of phenolic constituents in 17 varieties of cowpeas [J].
Cai, R ;
Hettiarachchy, NS ;
Jalaluddin, A .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2003, 51 (06) :1623-1627
[8]   Chemical components with health implications in wild and cultivated Mexican common bean seeds (Phaseolus vulgaris L.) [J].
Díaz-Batalla, L ;
Widholm, JM ;
Fahey, GC ;
Castaño-Tostado, E ;
Paredes-López, O .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2006, 54 (06) :2045-2052
[9]   Assessment of in vitro antioxidant capacity of the seed coat and the cotyledon of legumes in relation to their phenolic contents [J].
Dueñas, M ;
Hernández, T ;
Estrella, I .
FOOD CHEMISTRY, 2006, 98 (01) :95-103
[10]   Changes in the content of bioactive polyphenolic compounds of lentils by the action of exogenous enzymes.: Effect on their antioxidant activity [J].
Duenas, M. ;
Hernandez, T. ;
Estrella, I. .
FOOD CHEMISTRY, 2007, 101 (01) :90-97