Filimonas endophytica sp nov., isolated from surface-sterilized root of Cosmos bipinnatus

A Gram-stain-negative, yellow, motile by gliding, filamentous bacterium, designated SR 2-06(T), was isolated from surface-sterilized root of garden cosmos. 16S rRNA gene sequence analysis indicated that SR 2-06(T) was related most closely to Filimonas lacunae YT21(T) of the family Chitinophagaceae at a sequence similarity of 96.90 %, while levels of similarity to other related taxa were less than 93.08 %. Strain SR 2-06(T) exhibited similar features to F. lacunae in that it contained MK-7 as the major respiratory quinone, and iso-C-15 (:) (1) G, iso-C-15 (:) (0) and a summed feature consisting of C-16 (:) (1)omega 6c and/or C-16 (:) (1)omega 7c as the major fatty acids. However, strain SR 2-06(T) was distinguished from F. lacunae using a combination of physiological and biochemical properties. The cellular polar lipids were phosphatidylethanolamine, unknown aminophos-pholipids, unknown aminolipids, an unknown phospholipid and unidentified polar lipids. The DNA G+C content was 46.0 mol%. The phenotypic and phylogenetic evidence clearly indicates that strain SR 2-06(T) represents a novel species of the genus Filimonas, for which the name Filimonas endophytica sp. nov. is proposed. The type strain is SR 2-06(T) (=KCTC 42060(T)=JCM 19844(T)).