New Anti-Ku80 Monoclonal Antibody F10H2.B3 As a Useful Marker for Dividing Cells in Culture

被引：0

作者：

Mikelsaar, Aavo-Valdur ^{[1
,2
]}

Sunter, Alar ^{[1
]}

Toomik, Peeter ^{[1
]}

Karpson, Kalmer ^{[2
]}

Juronen, Erkki ^{[1
]}

机构：

[1] Univ Tartu, Inst Gen & Mol Pathol, EE-50411 Tartu, Estonia

[2] LabAs Ltd, Tartu, Estonia

来源：

HYBRIDOMA | 2009年 / 28卷 / 02期

关键词：

KU AUTOANTIGEN; DNA; PROTEIN; LOCALIZATION; SEQUENCE; REPAIR; ADHESION; RECEPTOR; SUBUNIT; COMPLEX;

D O I：

10.1089/hyb.2008.0077

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report on the development of a mouse monoclonal antibody (named F10H2.B3) using the native cellular fragments of human fetal neural stem cells as immunogens. Molecular analysis has shown that the target antigen of F10H2.B3 is Ku80 (ATP-dependent DNA helicase 2 subunit 2 [EC 3.6.1.-]). We suggest this antibody could be used in certain conditions as a proliferation marker for cells of different origin.

引用

页码：107 / 111

页数：5

共 27 条

[1] Interferon-α signaling promotes nucleus-to-cytoplasmic redistribution of p95Vav, and formation of a multisubunit complex involving Vav, Ku80, and Tyk2 [J].

Adam, L ;

Bandyopadhyay, D ;

Kumar, R .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 267 (03) :692-696

[2] Ku80 as a novel receptor for thymosin β4 that mediates its intracellular activity different from G-actin sequestering [J].

Bednarek, Radoslaw ;

Boncela, Joanna ;

Smolarczyk, Katarzyna ;

Cierniewska-Cieslak, Aleksandra ;

Wyroba, Elzbieta ;

Cierniewski, Czeslaw S. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2008, 283 (03) :1534-1544

[3] Evidence implicating Ku antigen as a structural factor in RNA polymerase II-mediated transcription [J].

Bertinato, J ;

Tomlinson, JJ ;

Schild-Poulter, C ;

Haché, RJG .

GENE, 2003, 302 (1-2) :53-64

[4] Sequence-specific DNA binding by Ku autoantigen and its effects on transcription [J].

Giffin, W ;

Torrance, H ;

Rodda, DJ ;

Prefontaine, GG ;

Pope, L ;

Hache, RJG .

NATURE, 1996, 380 (6571) :265-268

[5]

GROTE J, 2006, BMC MOL BIOL, V48, P1

[6] The biology of Ku and its potential oncogenic role in cancer [J].

Gullo, Charles ;

Au, Melvin ;

Feng, Ge ;

Teoh, Gerrard .

BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER, 2006, 1765 (02) :223-234

[7] Mechanism of DNA double-strand break repair by non-homologous end joining [J].

Hefferin, ML ;

Tomkinson, AE .

DNA REPAIR, 2005, 4 (06) :639-648

[8] Chemokine receptor CCR5 expression in in vitro differentiating human fetal neural stem/progenitor and glioblastoma cells [J].

Kalev, I ;

Kaasik, A ;

Zarkovski, A ;

Mikelsaar, AV .

NEUROSCIENCE LETTERS, 2006, 394 (01) :22-27

[9]

Koike M, 1999, J CELL SCI, V112, P4031

[10] Dimerization, translocation and localization of Ku70 and Ku80 proteins [J].

Koike, M .

JOURNAL OF RADIATION RESEARCH, 2002, 43 (03) :223-236

← 1 2 3 →