It's green outside: tracking cell surface proteins with pH-sensitive GFP

被引：97

作者：

Ashby, MC ^{[1
]}

Ibaraki, K ^{[1
]}

Henley, JM ^{[1
]}

机构：

[1] Univ Bristol, Sch Med Sci, Dept Anat, MRC Ctr Synapt Plast, Bristol BS8 1TD, Avon, England

来源：

TRENDS IN NEUROSCIENCES | 2004年 / 27卷 / 05期

基金：

英国医学研究理事会; 英国惠康基金;

关键词：

D O I：

10.1016/j.tins.2004.03.010

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Green fluorescent protein (GFP) and mutated GFP variants have proved to be immensely powerful tools that have had a profound impact on research in biological sciences. This review considers the development, use and future implications of pH-dependent GFP variants (e.g. pHluorins). These proteins hold considerable promise for the relatively non-invasive monitoring of events such as exocytosis, endocytosis and protein surface expression in living neurons with high spatial and temporal resolution.

引用

页码：257 / 261

页数：5

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