It's green outside: tracking cell surface proteins with pH-sensitive GFP

被引：96
作者：
Ashby, MC [1 ]
Ibaraki, K [1 ]
Henley, JM [1 ]
机构：
[1] Univ Bristol, Sch Med Sci, Dept Anat, MRC Ctr Synapt Plast, Bristol BS8 1TD, Avon, England
来源：
TRENDS IN NEUROSCIENCES | 2004年 / 27卷 / 05期
基金：
英国医学研究理事会; 英国惠康基金;
关键词：
D O I：
10.1016/j.tins.2004.03.010
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Green fluorescent protein (GFP) and mutated GFP variants have proved to be immensely powerful tools that have had a profound impact on research in biological sciences. This review considers the development, use and future implications of pH-dependent GFP variants (e.g. pHluorins). These proteins hold considerable promise for the relatively non-invasive monitoring of events such as exocytosis, endocytosis and protein surface expression in living neurons with high spatial and temporal resolution.
引用
收藏
页码：257 / 261
页数：5
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