Cryphonectria hypovirus 3, a virus species in the family hypoviridae with a single open reading frame

被引:74
作者
Smart, CD
Yuan, W
Foglia, R
Nuss, DL
Fulbright, DW
Hillman, BI [1 ]
机构
[1] Michigan State Univ, Dept Bot & Plant Pathol, E Lansing, MI 48824 USA
[2] Rutgers State Univ, Cook Coll, Dept Plant Pathol, New Brunswick, NJ 08901 USA
[3] Univ Maryland, Ctr Agr Biotechnol, College Pk, MD 20742 USA
关键词
D O I
10.1006/viro.1999.0039
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Isolate Grand Haven (GH) 2 is a naturally occurring isolate of the chestnut blight fungus, Cryphonectria parasitica, that is greatly reduced in virulence due to the presence of a double-stranded RNA virus. Unlike many other virus-infected, hypovirulent isolates, GH2 is not substantially reduced in pigmentation, conidiation, or laccase expression compared to its virus-free counterpart. The dsRNA genome of the GH2 virus was cloned, sequenced, and compared to hypovirulence-associated viruses of the family Hypoviridae. GH2 dsRNA is considerably smaller than previously characterized members of the family, 9.8 kb compared to 12.5-12.7 kb for other members. The genome organization of GH2 dsRNA reflected the substantial difference in genome size. Like other members of the family, one strand contained a poly(A)(+) tail at the 3' end and a long sequence with several minicistrons at the 5' end of the same strand. Only a single open reading frame (ORF) of 8622 nucleotides was predicted from deduced translations of the poly(A)(+)-containing strand, however. This contrasts with the two-ORF structures of previously characterized members. Analysis of the deduced ORF of GH2 dsRNA revealed putative proteinase, RNA polymerase, and helicase domains similar to those previously identified in confirmed members of the virus family Hypoviridae. GH2 dsRNA was more distantly related to Cryphonectria hypovirus (CHV) 1-EP713 and CHV2-NB58 than the latter two were to each other but has features in common with each of those viruses. We propose that the GH2 virus be included in this taxon as a member of the genus Hypovirus, representing a strain of a new species, CHV3, (C) 1999 Academic Press.
引用
收藏
页码:66 / 73
页数:8
相关论文
共 34 条
[1]  
Allemann C, 1999, MOL ECOL, V8, P843, DOI 10.1046/j.1365-294X.1999.00639.x
[2]  
Anagnostakis SL, 1992, ANN REPORT NO NUT GR, V83, P39
[3]   SITE-DIRECTED MUTATIONS IN THE POTYVIRUS HC-PRO GENE AFFECT HELPER COMPONENT ACTIVITY, VIRUS ACCUMULATION, AND SYMPTOM EXPRESSION IN INFECTED TOBACCO PLANTS [J].
ATREYA, CD ;
ATREYA, PL ;
THORNBURY, DW ;
PIRONE, TP .
VIROLOGY, 1992, 191 (01) :106-111
[4]  
BROWN MP, 1997, THESIS RUGERS U NEW
[5]   ATTENUATION OF FUNGAL VIRULENCE BY SYNTHETIC INFECTIOUS HYPOVIRUS TRANSCRIPTS [J].
CHEN, BS ;
CHOI, GH ;
NUSS, DL .
SCIENCE, 1994, 264 (5166) :1762-1764
[6]   Infectious cDNA clone of hypovirus CHV1-Euro7:: a comparative virology approach to investigate virus-mediated hypovirulence of the chestnut blight fungus Cryphonectria parasitica [J].
Chen, BS ;
Nuss, DL .
JOURNAL OF VIROLOGY, 1999, 73 (02) :985-992
[7]   A VIRAL GENE CONFERS HYPOVIRULENCE-ASSOCIATED TRAITS TO THE CHESTNUT BLIGHT FUNGUS [J].
CHOI, GH ;
NUSS, DL .
EMBO JOURNAL, 1992, 11 (02) :473-477
[8]   COTRANSLATIONAL AUTOPROTEOLYSIS INVOLVED IN GENE-EXPRESSION FROM A DOUBLE-STRANDED-RNA GENETIC ELEMENT ASSOCIATED WITH HYPOVIRULENCE OF THE CHESTNUT BLIGHT FUNGUS [J].
CHOI, GH ;
SHAPIRA, R ;
NUSS, DL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (04) :1167-1171
[9]   DIVERSITY OF CRYPHONECTRIA-PARASITICA HYPOVIRULENCE-ASSOCIATED DOUBLE-STRANDED RNAS WITHIN A CHESTNUT POPULATION IN NEW-JERSEY [J].
CHUNG, PH ;
BEDKER, PJ ;
HILLMAN, BI .
PHYTOPATHOLOGY, 1994, 84 (09) :984-990
[10]   PAPAIN-LIKE PROTEASE-P-29 AS A SYMPTOM DETERMINANT ENCODED BY A HYPOVIRULENCE-ASSOCIATED VIRUS OF THE CHESTNUT BLIGHT FUNGUS [J].
CRAVEN, MG ;
PAWLYK, DM ;
CHOI, GH ;
NUSS, DL .
JOURNAL OF VIROLOGY, 1993, 67 (11) :6513-6521