Intraarticular injection of dexamethasone promotes bone erosion in collagen-induced arthritis in mice through up-regulation of RANKL expression

BackgroundDexamethasone (DEX) is an effective therapeutic option commonly used in the treatment of many inflammatory diseases. However, DEX could impair proliferation or differentiation of osteoblasts, suggesting a pivotal role of DEX in bone destruction.ObjectiveTo investigate whether intraarticular injection of DEX could exacerbate bone erosion during CIA development.SettingCollagen-induced arthritis (CIA) mice were divided into PBS-treated and DEX-treated groups (n=5/group). Negative control group: DBA/1 mice (n=5) were used as age-matched, healthy, untreated controls.MethodCIA was induced in male DBA/1 mice. Intraarticular injected DEX (0.01mg/Kg, 10l) into the knee joint of CIA on Day 28, Day 35, Day 42 and Day 49 post the 1st immunization.ResultsThe severity of the arthritic disease was ameliorated in DEX-treated mice, accompanied by the decreased expression of IL-6, IL-8 and TNF-. However, DEX treatment accelerates bone erosion and osteoporosis during CIA development and triggers higher expression of RANKL, IL-17 in vitro and vivo.Main outcome measureThe effect of DEX on bone structure was analyzed using Haematoxylin & Eosin (H&E) staining and Micro-CT. The levels of receptor activator for nuclear factor- B ligand (RANKL) and osteoprotegerin (OPG) were investigated by real-time PCR, Western Blot and immunohistochemical analysis. RASFs were stimulated with Interleukin (IL)-1 and then treated with different concentrations of DEX for 72h.ConclusionIntraarticular injection of DEX could exacerbate bone erosion in CIA model via up-regulation of RANKL expression.